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PMID: 2550793 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Beta interferon subtype 1 induction by tumor necrosis factor.

Molecular and cellular biology ·Vol. 9 ·No. 7 ·1989-07-00 ·Pages 3037-42

Jacobsen H, Mestan J, Mittnacht S, Dieffenbach CW

Abstract

Tumor necrosis factor (TNF) induces an antiviral state in various cell lines. This antiviral state is quite similar to that established by interferon (IFN), e.g., TNF treatment of HEp-2 cells induces 2',5'-oligoadenylate synthetase activity. Both antiviral activity and synthetase induction are greatly reduced when TNF treatment occurs in the presence of a beta interferon subtype 1 (IFN-beta 1)-neutralizing antiserum. However, no one has yet directly demonstrated IFN-beta 1 induction, either as an antiviral activity in supernatants from TNF-treated cells or as IFN-specific mRNA by Northern (RNA) blot analysis. We have adopted a recently described in vitro DNA amplification protocol for the detection of specific RNAs. By applying this method to RNA from HEp-2 cells, we could demonstrate increased levels of IFN-beta 1-specific transcripts after TNF treatment. Dose response and kinetics of IFN-beta 1 induction coincided with the TNF-induced antiviral state. Nuclear run-on analysis showed enhanced transcriptional activity of the IFN-beta 1 gene in TNF-treated cells. Our data substantiate a role of IFN-beta 1 as mediator of the biological activity of TNF in HEp-2 cells.

MeSH Terms
Blotting, Northern Cells, Cultured DNA/biosynthesis,genetics DNA-Directed DNA Polymerase Gene Amplification Gene Expression Regulation Humans Interferon Type I/biosynthesis,genetics Kinetics Neutralization Tests Oligonucleotides RNA, Messenger/genetics Tumor Necrosis Factor-alpha/physiology Vesicular stomatitis Indiana virus/immunology
Chemicals
Interferon Type I Oligonucleotides RNA, Messenger Tumor Necrosis Factor-alpha DNA DNA-Directed DNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jacobsen H
Institute of Virus Research, German Cancer Research Center, Heidelberg, Federal Republic of Germany.
Mestan J
Mittnacht S
Dieffenbach C W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.7.3037-3042.1989
Published
1989-07-00
Pages
3037-42
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362772
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.7.3037-3042.1989 PMC Full Text View journal details
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