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PMID: 270686 Published · ppublish English Journal Article

Induction and decay of human fibroblast interferon mRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 10 ·1977-10-00 ·Pages 4415-9

Cavalieri RL, Havell EA, Vilcek J, Pestka S

Abstract

Polyadenylylated interferon mRNA, obtained from induced human fibroblasts, was quantitatively assayed by synthesis of biologically active human interferon in Xenopus laevis oocytes. The assay for interferon mRNA was used to distinguish between various hypotheses relating to interferon induction and biosynthesis. The data demonstrate that on induction with poly(I-poly(C) human fibroblasts accumulate interferon mRNA for 1-1.5 hr, after which time the mRNA is rapidly degraded with a half-life (t 1/2) of 18 min. Treatment of cells with cycloheximide prolongs the period of accumulation to 3 hr and decreases the rate of mRNA inactivation (t 1/2 = 49 min). Treatment with actinomycin D decreases the rate of inactivation still further (t 1/2 = 68 min). A comparison of cellular interferon synthesis with the relative amounts of interferon m RNA after simple induction or inductionin the presence of the inhibitors (superinduction) indicated a general correlation. Thus, on induction, the genes for interferon are activated to produce a transcript for a short time. The superinducing treatments prolong the period of accumulation and decrease the rate of degradation of this transcript.

MeSH Terms
Animals Female Fibroblasts/metabolism Half-Life Humans In Vitro Techniques Interferons/genetics Oocytes/metabolism Poly A/metabolism Protein Biosynthesis RNA, Messenger/genetics,metabolism Time Factors Xenopus
Chemicals
RNA, Messenger Poly A Interferons
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cavalieri R L
Havell E A
Vilcek J
Pestka S
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.10.4415
Published
1977-10-00
Pages
4415-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431953
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.10.4415 PMC Full Text View journal details
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