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PMID: 2550799 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of postreceptor signaling in the pheromone response pathway of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 9 ·No. 9 ·1989-09-00 ·Pages 3720-6

Blinder D, Jenness DD

Abstract

alpha-Factor pheromone inhibits division of yeast a cells. After prolonged exposure to alpha-factor, the cells adapt to the stimulus and resume cell division. The sst2 mutation is known to inhibit adaptation. This report examines adaptation in scg1 (also designated gpa1) and STE4Hpl (Hpl indicates haploid lethal) mutants that exhibit constitutive activation of the pheromone response pathway. Recovery of the STE4Hpl mutant was blocked by the sst2-1 mutation, whereas recovery of the scg1-7 mutant was not completely blocked by sst2-1. These results indicate that both SST2-dependent and -independent mechanisms regulate postreceptor events in the pheromone response pathway. Down regulation of receptors in response to alpha-factor was independent of the signal that was generated in the scg1 mutant.

MeSH Terms
Adaptation, Physiological Cell Division/drug effects Mating Factor Peptides/pharmacology Pheromones/pharmacology Receptors, Cell Surface/drug effects,physiology Receptors, Mating Factor Receptors, Peptide Saccharomyces cerevisiae/drug effects,genetics,physiology Signal Transduction/drug effects Transcription Factors
Chemicals
Peptides Pheromones Receptors, Cell Surface Receptors, Mating Factor Receptors, Peptide Transcription Factors Mating Factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Blinder D
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.
Jenness D D
References (45)
45 references, click to expand
  1. Comparison of dose-response curves for alpha factor-induced cell division arrest, agglutination, and projection formation of yeast cells. Implication for the mechanism of alpha factor action.
    J Biol Chem. 1983 Nov 25;258(22):13849-56 PMID: 6358212
  2. Saccharomyces cerevisiae nuclear fusion requires prior activation by alpha factor.
    Mol Cell Biol. 1986 Oct;6(10):3490-7 PMID: 3540592
  3. Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein.
    Mol Cell Biol. 1987 Jul;7(7):2316-28 PMID: 3302672
  4. Yeast cells recover from mating pheromone alpha factor-induced division arrest by desensitization in the absence of alpha factor destruction.
    J Biol Chem. 1984 Jan 25;259(2):1004-10 PMID: 6363399
  5. Regulation of transmembrane signaling by receptor phosphorylation.
    Cell. 1987 Mar 27;48(6):913-22 PMID: 3030559
  6. Macromolecule synthesis in temperature-sensitive mutants of yeast.
    J Bacteriol. 1967 May;93(5):1662-70 PMID: 5337848
  7. Cell interactions and regulation of cell type in the yeast Saccharomyces cerevisiae.
    Annu Rev Microbiol. 1983;37:623-60 PMID: 6357062
  8. Recovery of S. cerevisiae a cells from G1 arrest by alpha factor pheromone requires endopeptidase action.
    Cell. 1979 Nov;18(3):623-35 PMID: 391400
  9. Nucleotide sequences of STE2 and STE3, cell type-specific sterile genes from Saccharomyces cerevisiae.
    EMBO J. 1985 Oct;4(10):2643-8 PMID: 16453635
  10. The carboxy-terminal segment of the yeast alpha-factor receptor is a regulatory domain.
    Cell. 1988 Oct 21;55(2):221-34 PMID: 2844413
  11. The yeast SCG1 gene: a G alpha-like protein implicated in the a- and alpha-factor response pathway.
    Cell. 1987 Sep 25;50(7):1001-10 PMID: 3113738
  12. Down regulation of the alpha-factor pheromone receptor in S. cerevisiae.
    Cell. 1986 Aug 1;46(3):345-53 PMID: 3015412
  13. Mating-defective ste mutations are suppressed by cell division cycle start mutations in Saccharomyces cerevisiae.
    Mol Cell Biol. 1982 Sep;2(9):1052-63 PMID: 6757719
  14. Constitutive mutants in the yeast pheromone response: ordered function of the gene products.
    Cell. 1989 Feb 10;56(3):479-86 PMID: 2644047
  15. Conjugation in Saccharomyces cerevisiae.
    Annu Rev Cell Biol. 1988;4:429-57 PMID: 2848554
  16. Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone.
    J Cell Biol. 1980 Jun;85(3):811-22 PMID: 6993497
  17. Yeast peptide pheromones, a-factor and alpha-factor, activate a common response mechanism in their target cells.
    Cell. 1986 Dec 26;47(6):929-37 PMID: 3022943
  18. Recovery of Saccharomyces cerevisiae mating-type a cells from G1 arrest by alpha factor.
    J Bacteriol. 1977 May;130(2):766-74 PMID: 400792
  19. Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. II. Genetic analysis of nonmating mutants.
    Genetics. 1974 Feb;76(2):273-88 PMID: 4595644
  20. Saccharomyces cerevisiae mutants unresponsive to alpha-factor pheromone: alpha-factor binding and extragenic suppression.
    Mol Cell Biol. 1987 Apr;7(4):1311-9 PMID: 3037311
  21. Occurrence in Saccharomyces cerevisiae of a gene homologous to the cDNA coding for the alpha subunit of mammalian G proteins.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2140-4 PMID: 3031665
  22. Binding of alpha-factor pheromone to Saccharomyces cerevisiae a cells: dissociation constant and number of binding sites.
    Mol Cell Biol. 1986 Jan;6(1):318-20 PMID: 3023832
  23. The a-factor pheromone of Saccharomyces cerevisiae is essential for mating.
    Mol Cell Biol. 1988 Mar;8(3):1309-18 PMID: 3285180
  24. Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Aug;7(8):2680-90 PMID: 3313002
  25. Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones.
    Mol Cell Biol. 1982 Jan;2(1):11-20 PMID: 7050665
  26. Synchronization of haploid yeast cell cycles, a prelude to conjugation.
    Exp Cell Res. 1973 Jan;76(1):111-7 PMID: 4566309
  27. Phototransduction. A role for calcium in adaptation.
    Nature. 1988 Jul 7;334(6177):16-7 PMID: 2455232
  28. Alpha-factor structural gene mutations in Saccharomyces cerevisiae: effects on alpha-factor production and mating.
    Mol Cell Biol. 1985 Apr;5(4):787-96 PMID: 3887136
  29. Binding of alpha-factor pheromone to yeast a cells: chemical and genetic evidence for an alpha-factor receptor.
    Cell. 1983 Dec;35(2 Pt 1):521-9 PMID: 6360378
  30. Neither methylating nor demethylating enzymes are required for bacterial chemotaxis.
    Cell. 1985 Sep;42(2):683-90 PMID: 3928170
  31. Multiple regulation of STE2, a mating-type-specific gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Jun;6(6):2106-14 PMID: 3023919
  32. STE2 protein of Saccharomyces kluyveri is a member of the rhodopsin/beta-adrenergic receptor family and is responsible for recognition of the peptide ligand alpha factor.
    Proc Natl Acad Sci U S A. 1988 Jun;85(11):3855-9 PMID: 2836861
  33. Control of yeast cell type by the mating type locus. I. Identification and control of expression of the a-specific gene BAR1.
    J Mol Biol. 1981 Dec 5;153(2):305-21 PMID: 7040681
  34. Mutations in a gene encoding the alpha subunit of a Saccharomyces cerevisiae G protein indicate a role in mating pheromone signaling.
    Mol Cell Biol. 1988 Jun;8(6):2484-93 PMID: 3136318
  35. The STE2 gene product is the ligand-binding component of the alpha-factor receptor of Saccharomyces cerevisiae.
    J Biol Chem. 1988 Aug 5;263(22):10836-42 PMID: 2839507
  36. The STE4 and STE18 genes of yeast encode potential beta and gamma subunits of the mating factor receptor-coupled G protein.
    Cell. 1989 Feb 10;56(3):467-77 PMID: 2536595
  37. Alpha-factor enhancement of hybrid formation by protoplast fusion in Saccharomyces cerevisiae II.
    Curr Genet. 1986;10(12):943-5 PMID: 3329038
  38. GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction.
    Cell. 1987 Sep 25;50(7):1011-9 PMID: 3113739
  39. The selection of S. cerevisiae mutants defective in the start event of cell division.
    Genetics. 1980 Jul;95(3):561-77 PMID: 7002718
  40. A yeast operator overlaps an upstream activation site.
    Cell. 1987 Jul 31;50(3):369-77 PMID: 3301002
  41. Physiological characterization of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones.
    Mol Cell Biol. 1982 Jan;2(1):21-9 PMID: 7050666
  42. Role of STE genes in the mating factor signaling pathway mediated by GPA1 in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Sep;8(9):3777-83 PMID: 3065623
  43. The C-terminus of the S. cerevisiae alpha-pheromone receptor mediates an adaptive response to pheromone.
    Cell. 1988 Aug 26;54(5):609-20 PMID: 2842059
  44. Evidence the yeast STE3 gene encodes a receptor for the peptide pheromone a factor: gene sequence and implications for the structure of the presumed receptor.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1418-22 PMID: 3006051
  45. The yeast alpha-factor receptor: structural properties deduced from the sequence of the STE2 gene.
    Nucleic Acids Res. 1985 Dec 9;13(23):8463-75 PMID: 3001640
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.9.3720-3726.1989
Published
1989-09-00
Pages
3720-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362432
Subset
IM
Grants
NIGMS NIH HHS · GM34719 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.9.3720-3726.1989 PMC Full Text View journal details
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