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PMID: 2550802 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Micronuclear genome organization in Euplotes crassus: a transposonlike element is removed during macronuclear development.

Molecular and cellular biology ·Vol. 9 ·No. 9 ·1989-09-00 ·Pages 3793-807

Baird SE, Fino GM, Tausta SL, Klobutcher LA

Abstract

After mating, hypotrichous ciliated protozoa transform a set of their micronuclear chromosomes into thousands of short, linear DNA molecules that form the macronuclear genome. To examine micronuclear genome organization in the hypotrich Euplotes crassus, we have analyzed two cloned segments of micronuclear DNA as well as the macronuclear DNA molecules that are derived from them. E. crassus was found to display a number of features characteristic of other hypotrich genomes, including (i) clustering and close spacing of the precursors of macronuclear DNA molecules, (ii) the frequent occurrence of internal eliminated sequences within macronuclear precursors, (iii) overlapping macronuclear precursors, (iv) lack of telomeric repeats at the ends of macronuclear precursors, and (v) alternative processing of the micronuclear chromosome to yield multiple macronuclear DNA molecules. In addition, a moderately repetitive, transposonlike element that interrupts the precursors of two macronuclear DNA molecules has been identified and characterized. This transposonlike element, designated Tec1, is shown to be reproducibly removed from one of the macronuclear precursors during independent episodes of macronuclear development.

MeSH Terms
Animals Base Sequence Cell Nucleus/metabolism Ciliophora/genetics,growth & development DNA Transposable Elements DNA, Recombinant/isolation & purification Genes Molecular Sequence Data Repetitive Sequences, Nucleic Acid Restriction Mapping
Chemicals
DNA Transposable Elements DNA, Recombinant
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Baird S E
Department of Biochemistry, University of Connecticut Health Center, Farmington 06032.
Fino G M
Tausta S L
Klobutcher L A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.9.3793-3807.1989
Published
1989-09-00
Pages
3793-807
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362441
Subset
IM
Grants
NIGMS NIH HHS · GM-33277 · United States
Databases
GENBANK
M28498, M28499, M28500, M28501, M28502, M28503, M28504, M28505, M28506
External Links
PubMed source DOI: 10.1128/mcb.9.9.3793-3807.1989 PMC Full Text View journal details
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