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PMID: 368590 Published · ppublish English Journal Article

Close vicinity of IS1 integration sites in the leader sequence of the gal operon of E. coli.

Molecular & general genetics : MGG ·Vol. 167 ·No. 3 ·1979-01-02 ·Pages 235-41

Kühn S, Fritz HJ, Starlinger P

Abstract

Four insertions of IS1 in the leader sequence of the gal operon of E. coli have been analysed. Two of them occur at the same position, but in opposite orientations. The other two are inserted one nucleotide to one side and four nucleotides to the other side, respectively. In each case, nine base pairs of the leader sequence of the gal operon are duplicated directly, and are found flanking the termini of IS1 at its junction with the gal operon. These repeated sequences differ from each other as expected from the different insertion sites.

MeSH Terms
Base Sequence Coliphages/genetics DNA, Bacterial/analysis,genetics DNA, Viral/genetics Escherichia coli/genetics Galactose/genetics Operon Recombination, Genetic
Chemicals
DNA, Bacterial DNA, Viral Galactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kühn S
Fritz H J
Starlinger P
References (23)
23 references, click to expand
  1. DNA sequence at the integration sites of the insertion element IS1.
    Cell. 1978 Mar;13(3):411-8 PMID: 350411
  2. Polar mutations in lac, gal and phage lambda consist of a few IS-DNA sequences inserted with either orientation.
    Mol Gen Genet. 1972;119(3):223-31 PMID: 4567156
  3. Insertion mutations in the control region of the galactose operon of E. coli. II. Physical characterization of the mutations.
    Mol Gen Genet. 1972;115(3):266-76 PMID: 4555680
  4. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
    Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
  5. Cleavage of bacteriophage fl DNA by the restriction enzyme of Escherichia coli B.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3220-4 PMID: 4564208
  6. Relief of polarity in DNA-dependent cell-free synthesis of enzymes of the galactose operon of Escherichia coli.
    Mol Gen Genet. 1972;116(3):258-76 PMID: 4558646
  7. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
    FEBS Lett. 1977 Oct 15;82(2):314-6 PMID: 913604
  8. Studies with IS1 and IS4.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1187-92 PMID: 385222
  9. IS1 insertion generates duplication of a nine base pair sequence at its target site.
    Cell. 1978 Mar;13(3):419-26 PMID: 350412
  10. Insertion mutations in the control region of the Gal operon of E. coli. I. Biological characterization of the mutations.
    Mol Gen Genet. 1972;115(3):258-65 PMID: 4555679
  11. The 5'-terminal nucleotide sequence of galactose messenger ribonucleic acid of Escherichia coli.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4940-4 PMID: 4612533
  12. Cleavage specificity of the restriction endonuclease isolated from Haemophilus gallinarum (Hga I).
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3213-6 PMID: 269384
  13. Mutations in the galactose-operator.
    Mol Gen Genet. 1970;108(4):322-30 PMID: 4924696
  14. Two kinds of insertions in bacterial genes.
    Mol Gen Genet. 1972;119(3):191-206 PMID: 4567154
  15. Nucleotide sequence of the operator-promoter region of the galactose operon of Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):106-10 PMID: 319453
  16. Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):293-7 PMID: 1061131
  17. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  18. The relationship between function and DNA sequence in an intercistronic regulatory region in phage lambda.
    Nature. 1978 Mar 30;272(5652):414-23 PMID: 634366
  19. IS-elements in microorganisms.
    Curr Top Microbiol Immunol. 1976;75:111-52 PMID: 797531
  20. Nucleotide sequence of an insertion element, IS1.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):615-9 PMID: 273224
  21. 0 degree mutations in the galactose operon in E. coli. I. Genetic characterization.
    Mol Gen Genet. 1967;100(2):178-89 PMID: 4869989
  22. Suppression of polarity of insertion mutations within the gal operon of E. coli.
    Mol Gen Genet. 1977 Mar 16;151(3):295-304 PMID: 325374
  23. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267414
Published
1979-01-02
Pages
235-41
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267414
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