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PMID: 2551677 Published · ppublish English Journal Article

A membrane component of the endoplasmic reticulum that may be essential for protein translocation.

The EMBO journal ·Vol. 8 ·No. 8 ·1989-08-00 ·Pages 2225-9

Hartmann E, Wiedmann M, Rapoport TA

Abstract

We have purified a glycosylated, membrane-spanning protein of relative molecular mass approximately 34,000 (Mr approximately 34 K) from canine microsomes that appears to be essential for protein translocation across the endoplasmic reticulum (ER) as shown by the inhibitory action of antibodies directed against it and of monovalent Fab-fragments produced from them. The ER membrane contains at least as many molecules of the 34 K membrane protein as bound ribosomes. The protein can be detected immunologically in tissues of various organisms, indicating an universal function.

MeSH Terms
Animals Antibodies Biological Transport Blotting, Western Dogs Electrophoresis, Polyacrylamide Gel Endoplasmic Reticulum/metabolism Membrane Proteins/isolation & purification,metabolism Microsomes/metabolism Protein Sorting Signals/metabolism Proteins/metabolism Ribonucleoproteins/isolation & purification Signal Recognition Particle
Chemicals
Antibodies Membrane Proteins Protein Sorting Signals Proteins Ribonucleoproteins Signal Recognition Particle
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hartmann E
Zentralinstitut fuer Molekularbiologie der Akademie der Wissenschaften der DDR, Berlin-Buch, GDR.
Wiedmann M
Rapoport T A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-08-00
Pages
2225-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401152
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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