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PMID: 2552293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A 125-base-pair CEN6 DNA fragment is sufficient for complete meiotic and mitotic centromere functions in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 9 ·No. 8 ·1989-08-00 ·Pages 3342-9

Cottarel G, Shero JH, Hieter P, Hegemann JH

Abstract

Saccharomyces cerevisiae centromeres contain a conserved region ranging from 111 to 119 base pairs (bp) in length, which is characterized by the three conserved DNA elements CDEI, CDEII, and CDEIII. We isolated a 125-bp CEN6 DNA fragment (named ML CEN6) containing only these conserved elements and assayed it completely separated from its chromosomal context on circular minichromosomes and on a large linear chromosome fragment. The results show that this 125-bp CEN6 DNA fragment is by itself sufficient for complete mitotic and meiotic centromere functions.

MeSH Terms
Base Sequence Centromere/physiology Chromosomes/physiology Cloning, Molecular DNA Restriction Enzymes DNA, Fungal/physiology Meiosis Mitosis Molecular Sequence Data Nondisjunction, Genetic Plasmids Saccharomyces cerevisiae/cytology,genetics
Chemicals
DNA, Fungal DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cottarel G
Institut fuer Mikrobiologie und Molekularbiologie, Justus Liebig Universitaet, Giessen, Federal Republic of Germany.
Shero J H
Hieter P
Hegemann J H
References (24)
24 references, click to expand
  1. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.
    Cell. 1984 May;37(1):67-75 PMID: 6373014
  2. Isolation of a yeast centromere and construction of functional small circular chromosomes.
    Nature. 1980 Oct 9;287(5782):504-9 PMID: 6999364
  3. Meiotic Diploid Progeny and Meiotic Nondisjunction in SACCHAROMYCES CEREVISIAE.
    Genetics. 1982 May;101(1):17-33 PMID: 17246080
  4. Mutational analysis of centromere DNA from chromosome VI of Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Jun;8(6):2523-35 PMID: 3043181
  5. The structure and function of yeast centromeres.
    Annu Rev Genet. 1985;19:29-55 PMID: 3909945
  6. Structure and sequence of the centromeric DNA of chromosome 4 in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Jan;6(1):241-5 PMID: 3537685
  7. Functional selection and analysis of yeast centromeric DNA.
    Cell. 1985 Oct;42(3):913-21 PMID: 2996783
  8. Genetic manipulation of centromere function.
    Mol Cell Biol. 1987 Jul;7(7):2397-405 PMID: 3302676
  9. Genomic substitutions of centromeres in Saccharomyces cerevisiae.
    Nature. 1983 Sep 1-7;305(5929):23-8 PMID: 6350891
  10. Mutational and in vitro protein-binding studies on centromere DNA from Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Dec;7(12):4522-34 PMID: 2830498
  11. Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss.
    Cell. 1985 Feb;40(2):381-92 PMID: 3967296
  12. Yeast centromere DNA is in a unique and highly ordered structure in chromosomes and small circular minichromosomes.
    Cell. 1982 Jun;29(2):305-17 PMID: 6288253
  13. Alterations in the adenine-plus-thymine-rich region of CEN3 affect centromere function in Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Jan;7(1):68-75 PMID: 3550426
  14. Structural and functional analysis of a yeast centromere (CEN3).
    J Cell Sci Suppl. 1984;1:43-58 PMID: 6397474
  15. Yeast centromeres.
    Yeast. 1987 Sep;3(3):187-200 PMID: 3332973
  16. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  17. Single base-pair mutations in centromere element III cause aberrant chromosome segregation in Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Feb;6(2):530-8 PMID: 3537689
  18. A consensus transcription termination sequence in the promoter region is necessary for efficient gene expression of the TRP1 gene of Saccharomyces cerevisiae.
    Mol Gen Genet. 1988 Jun;212(3):495-504 PMID: 3047551
  19. Role of conserved sequence elements in yeast centromere DNA.
    EMBO J. 1985 Jul;4(7):1867-74 PMID: 2992949
  20. Mutations in the right boundary of Saccharomyces cerevisiae centromere 6 lead to nonfunctional or partially functional centromeres.
    Mol Gen Genet. 1986 Nov;205(2):305-11 PMID: 3027507
  21. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  22. Nucleotide sequence comparisons and functional analysis of yeast centromere DNAs.
    Cell. 1982 May;29(1):235-44 PMID: 7049398
  23. Mutational analysis of meiotic and mitotic centromere function in Saccharomyces cerevisiae.
    Genetics. 1987 Oct;117(2):203-12 PMID: 3311877
  24. Centromeric DNA from chromosome VI in Saccharomyces cerevisiae strains.
    EMBO J. 1982;1(12):1605-11 PMID: 6327282
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.8.3342-3349.1989
Published
1989-08-00
Pages
3342-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362379
Subset
IM
Grants
NCI NIH HHS · 5P01CA16519-13 · United States
Databases
GENBANK
X01855
External Links
PubMed source DOI: 10.1128/mcb.9.8.3342-3349.1989 PMC Full Text View journal details
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