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PMID: 3047551 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A consensus transcription termination sequence in the promoter region is necessary for efficient gene expression of the TRP1 gene of Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 212 ·No. 3 ·1988-06-00 ·Pages 495-504

Braus G, Paravicini G, Hütter R

Abstract

The TRP1 gene of Saccharomyces cerevisiae is the only TRP gene which is not derepressible by the general control regulatory system. In the TRP1 promoter transcription starts at five initiation sites, organized in two clusters. The two transcripts of the first, more upstream cluster include a long leader sequence of approximately 200 bp. A transcriptional terminator element located in the 5' region of the TRP1 gene is essential for accurate gene expression. In partial TRP1 promoters lacking the terminator, like the original EcoRI TRP1 fragment used in numerous vectors, plasmid-encoded transcription is initiated predominantly in adjacent vector regions, resulting mainly in large, poorly translated transcripts. This poor translation is not due to mRNA instability. The effect can be suppressed by introducing artificial transcription barriers between vector sequences and the truncated EcoRI TRP1 fragment.

MeSH Terms
Aldose-Ketose Isomerases Base Sequence Carbohydrate Epimerases/metabolism Chromosome Mapping Cloning, Molecular Gene Expression Regulation Genes, Fungal Genes, Regulator Nucleic Acid Hybridization Plasmids Promoter Regions, Genetic Saccharomyces cerevisiae/genetics Terminator Regions, Genetic Transcription, Genetic Transformation, Genetic
Chemicals
Carbohydrate Epimerases Aldose-Ketose Isomerases phosphoribosylanthranilate isomerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Braus G
Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH-Zentum, Zürich, Switzerland.
Paravicini G
Hütter R
References (64)
64 references, click to expand
  1. Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Aug;4(8):1440-8 PMID: 6092912
  2. Multiple upstream AUG codons mediate translational control of GCN4.
    Cell. 1986 Apr 25;45(2):201-7 PMID: 3516411
  3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  4. The enzymatic conversion of anthranilate to indolylglycerol phosphate in Neurospora crassa.
    J Biol Chem. 1965 Oct;240(10):3781-8 PMID: 5842052
  5. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 PMID: 375221
  6. High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3829-33 PMID: 386351
  7. A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast.
    Cell. 1983 Jan;32(1):89-98 PMID: 6337724
  8. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  9. Permeabilization of microorganisms by Triton X-100.
    Anal Biochem. 1978 Oct 1;90(1):220-33 PMID: 365019
  10. Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast.
    Methods Enzymol. 1983;100:293-308 PMID: 6312261
  11. Regulation of tryptophan biosynthesis in Saccharomyces cerevisiae: mode of action of 5-methyl-tryptophan and 5-methyl-tryptophan-sensitive mutants.
    J Bacteriol. 1974 Mar;117(3):1131-40 PMID: 4360539
  12. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  13. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  14. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  15. Promotor mutants of the yeast his3 gene.
    J Mol Biol. 1981 Nov 5;152(3):553-68 PMID: 6173490
  16. Structure and function of the TRP3 gene of Saccharomyces cerevisiae: Analysis of transcription, promoter sequence, and sequence coding for a glutamine amidotransferase.
    Curr Genet. 1984 Apr;8(3):165-72 PMID: 24177735
  17. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  18. Isolation of the TRP2 and the TRP3 genes of Saccharomyces cerevisiae by functional complementation in yeast.
    Curr Genet. 1982 May;5(1):39-46 PMID: 24186086
  19. The yeast his3 promoter contains at least two distinct elements.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7385-9 PMID: 6760196
  20. The nucleotide sequence of the HIS4 region of yeast.
    Gene. 1982 Apr;18(1):47-59 PMID: 7049842
  21. Expression of an artificial yeast TRP-gene cluster in yeast and Escherichia coli.
    Mol Gen Genet. 1984;195(3):481-6 PMID: 6381966
  22. Identification of sequences in a yeast histone promoter involved in periodic transcription.
    Cell. 1986 May 23;45(4):537-44 PMID: 3518945
  23. Local protein-DNA interactions may determine nucleosome positions on yeast plasmids.
    Nature. 1985 May 16-22;315(6016):250-2 PMID: 3889654
  24. Nucleotide sequence of Saccharomyces cerevisiae genes TRP2 and TRP3 encoding bifunctional anthranilate synthase: indole-3-glycerol phosphate synthase.
    J Biol Chem. 1984 Mar 25;259(6):3985-92 PMID: 6323449
  25. Tryptophan biosynthesis in Saccharomyces cerevisiae: control of the flux through the pathway.
    J Bacteriol. 1978 Apr;134(1):48-59 PMID: 348687
  26. Each of three "TATA elements" specifies a subset of the transcription initiation sites at the CYC-1 promoter of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8562-6 PMID: 3001709
  27. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli.
    Plasmid. 1984 Jul;12(1):19-36 PMID: 6387733
  28. Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene.
    Gene. 1980 Jul;10(2):157-66 PMID: 6248420
  29. Yeast cytochrome c messenger RNA. In vitro translation and specific immunoprecipitation of the CYC1 gene product.
    J Biol Chem. 1976 Oct 25;251(20):6320-6 PMID: 185210
  30. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  31. Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins.
    Nature. 1985 Aug 29-Sep 4;316(6031):774-8 PMID: 4041012
  32. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  33. GCN4 protein, synthesized in vitro, binds HIS3 regulatory sequences: implications for general control of amino acid biosynthetic genes in yeast.
    Cell. 1985 Nov;43(1):177-88 PMID: 3907851
  34. Transformation of yeast by a replicating hybrid plasmid.
    Nature. 1978 Sep 14;275(5676):104-9 PMID: 357984
  35. Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast trpl region.
    Gene. 1979 Oct;7(2):141-52 PMID: 389741
  36. Initiation of translation at internal AUG codons in mammalian cells.
    Nature. 1984 May 3-9;309(5963):82-5 PMID: 6717585
  37. Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2199-203 PMID: 6264467
  38. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  39. Constitutive and coordinately regulated transcription of yeast genes: promoter elements, positive and negative regulatory sites, and DNA binding proteins.
    Cold Spring Harb Symp Quant Biol. 1985;50:489-503 PMID: 3913566
  40. Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
    Nucleic Acids Res. 1979 Nov 10;7(5):1175-93 PMID: 390497
  41. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  42. The yeast PHO5 promoter: phosphate-control elements and sequences mediating mRNA start-site selection.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1340-4 PMID: 2881299
  43. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  44. Structural analysis of the two tandemly repeated acid phosphatase genes in yeast.
    Nucleic Acids Res. 1984 Oct 25;12(20):7721-39 PMID: 6093051
  45. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  46. Modulator sequences mediate oxygen regulation of CYC1 and a neighboring gene in yeast.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):151-5 PMID: 6296862
  47. The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper.
    Anal Biochem. 1980 Nov 15;109(1):123-9 PMID: 6162401
  48. The TRP4 gene of Saccharomyces cerevisiae: isolation and structural analysis.
    Nucleic Acids Res. 1986 Aug 26;14(16):6357-73 PMID: 2428012
  49. Multiple control elements in the TRP1 promoter of Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Dec;6(12):4251-8 PMID: 3025648
  50. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  51. Expression in Saccharomyces cerevisiae of human interferon-alpha directed by the TRP1 5' region.
    Nucleic Acids Res. 1983 Apr 25;11(8):2287-302 PMID: 6304646
  52. Arrangement of genes TRP1 and TRP3 of Saccharomyces cerevisiae strains.
    Arch Microbiol. 1985 Sep;142(4):383-8 PMID: 2998296
  53. The nonenzymatic preparation in solution of N-(5'-phosphoribosyl) anthranilic acid, an intermediate in tryptophan biosynthesis.
    J Biol Chem. 1968 Nov 10;243(21):5605-9 PMID: 5699054
  54. Free tryptophan pool and tryptophan biosynthetic enzymes in Saccharomyces cerevisiae.
    Arch Microbiol. 1976 Mar 19;107(2):207-14 PMID: 769720
  55. Vector systems for the expression, analysis and cloning of DNA sequences in S. cerevisiae.
    Yeast. 1985 Dec;1(2):83-138 PMID: 3916863
  56. The general control of amino acid biosynthetic genes in the yeast Saccharomyces cerevisiae.
    CRC Crit Rev Biochem. 1986;21(3):277-317 PMID: 3536302
  57. Calcium-dependent bacteriophage DNA infection.
    J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
  58. Yeast mRNA initiation sites are determined primarily by specific sequences, not by the distance from the TATA element.
    EMBO J. 1985 Dec 1;4(12):3273-80 PMID: 3912167
  59. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  60. Construction, replication, and chromatin structure of TRP1 RI circle, a multiple-copy synthetic plasmid derived from Saccharomyces cerevisiae chromosomal DNA.
    Mol Cell Biol. 1982 Mar;2(3):221-32 PMID: 6287231
  61. Yeast gene TRP5: structure, function, regulation.
    J Biol Chem. 1982 Feb 10;257(3):1491-500 PMID: 6276387
  62. Affecting gene expression by altering the length and sequence of the 5' leader.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7698-702 PMID: 6096850
  63. Transcription terminator-like element within a Saccharomyces cerevisiae promoter region.
    Mol Cell Biol. 1986 Apr;6(4):1095-101 PMID: 3023868
  64. Centromeric DNA from chromosome VI in Saccharomyces cerevisiae strains.
    EMBO J. 1982;1(12):1605-11 PMID: 6327282
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330855
Published
1988-06-00
Pages
495-504
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330855
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