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PMID: 2554303 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The mRNA encoding a high-affinity cAMP phosphodiesterase is regulated by hormones and cAMP.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 21 ·1989-11-00 ·Pages 8197-201

Swinnen JV, Joseph DR, Conti M

Abstract

To elucidate the mechanisms by which hormones regulate cAMP phosphodiesterases (PDEs), a group of cDNA clones that had been isolated from a rat Sertoli cell library were characterized. These cDNAs are derived from a single gene (ratPDE3). The deduced amino acid sequence of the ratPDE3 cDNA corresponds to a 66,200-Da protein homologous to other testicular PDEs, to the Drosophila melanogaster dunce-encoded cAMP PDE, and to bovine and yeast PDEs. Expression of ratPDE3 in eukaryotic and prokaryotic cells leads to the appearance of a cAMP PDE with properties identical to the cAMP PDE purified from Sertoli cells. Although of different size, transcripts corresponding to ratPDE3 were present in all organs studied. In the immature Sertoli cell in culture, the level of mRNA transcripts of ratPDE3 was increased more than 100-fold by follicle-stimulating hormone or N6,O2'-dibutyryladenosine 3',5'-cyclic monophosphate treatment. Stimulation of ratPDE3 mRNA by N6,O2'-dibutyryladenosine 3',5'-cyclic monophosphate was also observed in a C6 glioma cell line. These data demonstrate that cAMP regulates the expression of one of its own degrading enzymes by an intracellular feedback mechanism that involves changes in mRNA levels.

MeSH Terms
3',5'-Cyclic-AMP Phosphodiesterases/genetics,metabolism Amino Acid Sequence Animals Base Sequence Bucladesine/pharmacology Cell Line Cells, Cultured Cyclic AMP/physiology DNA/genetics,isolation & purification Follicle Stimulating Hormone/pharmacology Gene Expression Regulation, Enzymologic/drug effects Gene Library Genes/drug effects Glioma Kinetics Male Molecular Sequence Data RNA, Messenger/genetics Rats Rats, Inbred Strains Sertoli Cells/enzymology Transcription, Genetic/drug effects Transfection
Chemicals
RNA, Messenger Bucladesine Follicle Stimulating Hormone DNA Cyclic AMP 3',5'-Cyclic-AMP Phosphodiesterases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Swinnen J V
Department of Pediatrics, University of North Carolina, Chapel Hill 27599.
Joseph D R
Conti M
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.21.8197
Published
1989-11-00
Pages
8197-201
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298247
Subset
IM
Grants
NICHD NIH HHS · HD20788 · United States
NICHD NIH HHS · P30-HD18968 · United States
Databases
GENBANK
M25349
External Links
PubMed source DOI: 10.1073/pnas.86.21.8197 PMC Full Text View journal details
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