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PMID: 2554308 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell proteins bind to multiple sites within the 5' untranslated region of poliovirus RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 21 ·1989-11-00 ·Pages 8299-303

del Angel RM, Papavassiliou AG, Fernández-Tomás C, Silverstein SJ, Racaniello VR

Abstract

The 5' noncoding region of poliovirus RNA contains sequences necessary for translation and replication. These functions are probably carried out by recognition of poliovirus RNA by cellular and/or viral proteins. Using a mobility-shift electrophoresis assay and 1,10-phenanthroline/Cu+ footprinting, we demonstrate specific binding of cytoplasmic factors with a sequence from nucleotides 510-629 within the 5' untranslated region (UTR). Complex formation was also observed with a second sequence (nucleotides 97-182) within the 5' UTR. These two regions of the 5' UTR appear to be recognized by distinct cell factors as determined by competition analysis and the effects of ionic strength on complex formation. However, both complexes contain eukaryotic initiation factor 2 alpha, as revealed by their reaction with specific antibody.

MeSH Terms

HeLa Cells/metabolism Humans Neoplasm Proteins/metabolism Nucleotide Mapping Poliovirus/genetics Protein Binding RNA, Viral/biosynthesis,metabolism Templates, Genetic

Chemicals

Neoplasm Proteins RNA, Viral

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

del Angel R M

Departmento de Genética y Biologia Molecular, Centro de Investigación y de Estudios Avanzados, Mexico City, Distrito Federal.

Papavassiliou A G

Fernández-Tomás C

Silverstein S J

Racaniello V R

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.86.21.8299

Published

1989-11-00

Pages

8299-303

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC298268

Subset

Grants

NIGMS NIH HHS · GM38125 · United States

External Links

PubMed source DOI: 10.1073/pnas.86.21.8299 PMC Full Text View journal details

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Cell proteins bind to multiple sites within the 5' untranslated region of poliovirus RNA.

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