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PMID: 2555697 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of an upstream activation sequence and other cis-acting elements required for transcription of COX6 from Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 9 ·No. 12 ·1989-12-00 ·Pages 5350-8

Trawick JD, Rogness C, Poyton RO

Abstract

Transcription of Saccharomyces cerevisiae COX6, the nuclear gene for subunit VI of cytochrome c oxidase, is activated in heme-proficient cells, requires the HAP2 gene, and is subject to glucose repression. In this study, by deletion mutagenesis of the COX6 promoter, we identified two regions that are important for transcription. The first was an upstream activation site, UAS6. It was found to be contained within an 84-base-pair (bp) sequence, between bp -256 and -340 of the COX6 translational initiation codon, and to contain sequences required for activation by heme and HAP2 and for release from glucose repression. When located upstream of a CYC1-lacZ fusion gene, deleted for both of its UASs, this segment functioned as a UAS element. Although UAS6 could promote expression in either orientation, it showed a marked orientation dependence in its response to HAP2 and the carbon source. The second region lay between bp -255 and -91. It contained two of the three major 5' termini of COX6 mRNAs and a putative TATA box. Deletion analysis of this region demonstrated that the putative TATA box is not required for transcription and that this region is separable into two redundant domains.

MeSH Terms
Chromosome Deletion Electron Transport Complex IV/genetics Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Genes, Fungal Macromolecular Substances Mutation Plasmids Promoter Regions, Genetic RNA, Messenger/genetics Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Transcription, Genetic beta-Galactosidase/genetics,metabolism
Chemicals
Macromolecular Substances RNA, Messenger Electron Transport Complex IV beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Trawick J D
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
Rogness C
Poyton R O
References (48)
48 references, click to expand
  1. Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2199-203 PMID: 6264467
  2. Organization and expression of the COX6 genetic locus in Saccharomyces cerevisiae: multiple mRNAs with different 3' termini are transcribed from COX6 and regulated differentially.
    Nucleic Acids Res. 1989 Feb 11;17(3):1103-20 PMID: 2537949
  3. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  4. The biogenesis of mitochondria in microorganisms.
    Annu Rev Microbiol. 1972;26:163-98 PMID: 4264317
  5. Derepression of mitochondria and their enzymes in yeast: regulatory aspects.
    Arch Biochem Biophys. 1974 May;162(1):248-71 PMID: 4151576
  6. Regulation of mitochondrial biogenesis: enzymatic changes in cytochrome-deficient yeast mutants requiring delta-aminolevulinic acid.
    J Biol Chem. 1975 Dec 10;250(23):9090-8 PMID: 127792
  7. Yeast mutants deficient in heme biosynthesis and a heme mutant additionally blocked in cyclization of 2,3-oxidosqualene.
    J Biol Chem. 1977 May 10;252(9):2846-54 PMID: 323256
  8. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  9. Kinetics of glucose repression of yeast cytochrome c.
    J Bacteriol. 1978 Jul;135(1):39-44 PMID: 209012
  10. Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae.
    Cell. 1979 Apr;16(4):753-61 PMID: 222467
  11. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.
    Anal Biochem. 1979 Oct 1;98(2):305-9 PMID: 386835
  12. Modulator sequences mediate oxygen regulation of CYC1 and a neighboring gene in yeast.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):151-5 PMID: 6296862
  13. Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.
    Cell. 1983 Apr;32(4):1279-86 PMID: 6301690
  14. Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast.
    Methods Enzymol. 1983;101:181-91 PMID: 6310321
  15. Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae.
    Cell. 1984 Feb;36(2):503-11 PMID: 6319028
  16. The nuclear-coded subunits of yeast cytochrome c oxidase. I. Fractionation of the holoenzyme into chemically pure polypeptides and the identification of two new subunits using solvent extraction and reversed phase high performance liquid chromatography.
    J Biol Chem. 1984 May 25;259(10):6564-70 PMID: 6327684
  17. Glucose represses transcription of Saccharomyces cerevisiae nuclear genes that encode mitochondrial components.
    Mol Cell Biol. 1984 May;4(5):939-46 PMID: 6328277
  18. Isolation and sequence of the structural gene for cytochrome c oxidase subunit VI from Saccharomyces cerevisiae.
    J Biol Chem. 1984 Dec 25;259(24):15401-7 PMID: 6210289
  19. Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the "TATA box".
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7860-4 PMID: 6096863
  20. Subunit IV of yeast cytochrome c oxidase: cloning and nucleotide sequencing of the gene and partial amino acid sequencing of the mature protein.
    EMBO J. 1984 Dec 1;3(12):2831-7 PMID: 6098449
  21. Two nonidentical forms of subunit V are functional in yeast cytochrome c oxidase.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2235-9 PMID: 2986105
  22. Regulation of the expression of iso 2-cytochrome c gene in S. cerevisiae: cloning of the positive regulatory gene CYP1 and identification of the region of its target sequence on the structural gene CYP3.
    Mol Gen Genet. 1985;199(3):524-33 PMID: 2993801
  23. Nucleotide sequence and transcriptional mapping of the yeast pet56-his3-ded1 gene region.
    Nucleic Acids Res. 1985 Dec 9;13(23):8587-601 PMID: 3001645
  24. The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal.
    J Cell Biol. 1986 Feb;102(2):523-33 PMID: 2868014
  25. Cloning and molecular analysis of the HAP2 locus: a global regulator of respiratory genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1985 Dec;5(12):3410-6 PMID: 3915775
  26. Characterization of COX9, the nuclear gene encoding the yeast mitochondrial protein cytochrome c oxidase subunit VIIa. Subunit VIIa lacks a leader peptide and is an essential component of the holoenzyme.
    J Biol Chem. 1986 Dec 25;261(36):17183-91 PMID: 3023385
  27. COX8, the structural gene for yeast cytochrome c oxidase subunit VIII. DNA sequence and gene disruption indicate that subunit VIII is required for maximal levels of cellular respiration and is derived from a precursor which is extended at both its NH2 and COOH termini.
    J Biol Chem. 1986 Dec 25;261(36):17192-7 PMID: 3023386
  28. Tandemly duplicated upstream control sequences mediate copper-induced transcription of the Saccharomyces cerevisiae copper-metallothionein gene.
    Mol Cell Biol. 1986 Apr;6(4):1158-63 PMID: 3537699
  29. Efficient expression of the Saccharomyces cerevisiae PGK gene depends on an upstream activation sequence but does not require TATA sequences.
    Mol Cell Biol. 1986 Dec;6(12):4335-43 PMID: 3540610
  30. Yeast HAP1 activator competes with the factor RC2 for binding to the upstream activation site UAS1 of the CYC1 gene.
    Cell. 1987 Apr 10;49(1):9-18 PMID: 3030567
  31. Transcription of yeast COX6, the gene for cytochrome c oxidase subunit VI, is dependent on heme and on the HAP2 gene.
    J Biol Chem. 1989 Apr 25;264(12):7005-8 PMID: 2540169
  32. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  33. Promoters, activator proteins, and the mechanism of transcriptional initiation in yeast.
    Cell. 1987 May 8;49(3):295-7 PMID: 2882858
  34. The untranslated leader of nuclear COX4 gene of Saccharomyces cerevisiae contains an intron.
    Nucleic Acids Res. 1987 Apr 24;15(8):3515-29 PMID: 3033605
  35. Characterization of the yeast HEM2 gene and transcriptional regulation of COX5 and COR1 by heme.
    J Biol Chem. 1987 Dec 15;262(35):16822-9 PMID: 2445751
  36. Structural analysis of two genes encoding divergent forms of yeast cytochrome c oxidase subunit V.
    Mol Cell Biol. 1987 Oct;7(10):3511-9 PMID: 2824989
  37. Yeast HAP2 and HAP3 activators both bind to the CYC1 upstream activation site, UAS2, in an interdependent manner.
    Cell. 1987 Dec 24;51(6):953-61 PMID: 2826015
  38. Constitutive expression of the yeast HEM1 gene is actually a composite of activation and repression.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9113-7 PMID: 3321068
  39. UASs and enhancers: common mechanism of transcriptional activation in yeast and mammals.
    Cell. 1988 Feb 12;52(3):303-5 PMID: 2894251
  40. Regulatory proteins in yeast.
    Annu Rev Genet. 1987;21:425-52 PMID: 3327472
  41. Mutational analysis of upstream activation sequence 2 of the CYC1 gene of Saccharomyces cerevisiae: a HAP2-HAP3-responsive site.
    Mol Cell Biol. 1988 Feb;8(2):647-54 PMID: 2832731
  42. Demarcation of a sequence involved in mediating catabolite repression of the gene for the 11 kDa subunit VIII of ubiquinol-cytochrome c oxidoreductase in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1988 Jul 11;16(13):5797-811 PMID: 2840633
  43. Oxygen-dependent upstream activation sites of Saccharomyces cerevisiae cytochrome c genes are related forms of the same sequence.
    Mol Cell Biol. 1988 Jun;8(6):2275-9 PMID: 2841577
  44. Identification of two factors which bind to the upstream sequences of a number of nuclear genes coding for mitochondrial proteins and to genetic elements important for cell division in yeast.
    Nucleic Acids Res. 1988 Aug 11;16(15):7287-301 PMID: 3045755
  45. Co-ordinate control of synthesis of mitochondrial and non-mitochondrial hemoproteins: a binding site for the HAP1 (CYP1) protein in the UAS region of the yeast catalase T gene (CTT1).
    EMBO J. 1988 Jun;7(6):1799-804 PMID: 2844525
  46. Divergent promoters, a common form of gene organization.
    Microbiol Rev. 1988 Sep;52(3):318-26 PMID: 3054465
  47. Differential regulation of the two genes encoding Saccharomyces cerevisiae cytochrome c oxidase subunit V by heme and the HAP2 and REO1 genes.
    Mol Cell Biol. 1988 Oct;8(10):4537-40 PMID: 2847035
  48. Regulation of the nuclear-coded peptides of yeast cytochrome c oxidase.
    Biochemistry. 1982 Jan 19;21(2):309-16 PMID: 6280749
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.12.5350-5358.1989
Published
1989-12-00
Pages
5350-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363703
Subset
IM
Grants
NIGMS NIH HHS · GM 34827 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.12.5350-5358.1989 PMC Full Text View journal details
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