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PMID: 3045755 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of two factors which bind to the upstream sequences of a number of nuclear genes coding for mitochondrial proteins and to genetic elements important for cell division in yeast.

Nucleic acids research ·Vol. 16 ·No. 15 ·1988-08-11 ·Pages 7287-301

Dorsman JC, van Heeswijk WC, Grivell LA

Abstract

Two abundant factors, GFI and GFII which interact with the 5' flanking regions of nuclear genes coding for proteins of the mitochondrial respiratory chain have been identified. In one case (subunit VIII of QH2: cytochrome c oxidoreductase) the binding sites for both factors overlap completely and their binding is mutually exclusive. For the other 5' regions tested the GFI and GFII binding sites do not coincide. Interestingly, binding sites for GFI and GFII are also present in or at the 3' ends of the coding regions of two genes of the PHO gene family and in DNA elements important for optimal ARS and CEN function respectively. The sites recognized by GFI conform to the consensus RTCRNNNNNNACGNR, while those recognized by GFII contain the element RTCACGTG. We speculate that GFI and GFII may play a role in different cellular processes, dependent on the context of their binding sites and that one of these processes may be the coordination of the expression of genes involved in mitochondrial biogenesis with the progress of the cell cycle.

MeSH Terms

Base Sequence Cytochrome Reductases/genetics DNA Replication DNA-Binding Proteins/physiology Genes, Fungal NADH Dehydrogenase/genetics Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid Structure-Activity Relationship Submitochondrial Particles/enzymology Transcription Factors/physiology

Chemicals

DNA-Binding Proteins Transcription Factors Cytochrome Reductases NADH Dehydrogenase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Dorsman J C

Section for Molecular Biology, Department of Molecular Cell Biology, Amsterdam, The Netherlands.

van Heeswijk W C

Grivell L A

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.15.7287

Published

1988-08-11

Pages

7287-301

Language

English

Region

England

NLM ID

0411011

PMCID

PMC338409

Subset

External Links

PubMed source DOI: 10.1093/nar/16.15.7287 PMC Full Text View journal details

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Identification of two factors which bind to the upstream sequences of a number of nuclear genes coding for mitochondrial proteins and to genetic elements important for cell division in yeast.

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