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PMID: 3550420 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor.

Molecular and cellular biology ·Vol. 7 ·No. 1 ·1987-01-00 ·Pages 403-9

Bram RJ, Kornberg RD

Abstract

A protein that binds specifically to Saccharomyces cerevisiae centromere DNA element I was purified on the basis of a nitrocellulose filter-binding assay. This protein, termed centromere-binding protein 1 (CP1), was heat stable and renaturable from sodium dodecyl sulfate (SDS), and assays of eluates from SDS gels indicated a molecular weight of 57,000 to 64,000. An activity with similar specificity and stability was detected in human lymphocyte extracts, and analysis in SDS gels revealed a molecular weight of 39,000 to 49,000. CP1-binding sites occurred not only at centromeres but also near many transcription units, for example, adjacent to binding sites for the GAL4-positive regulatory protein upstream of the GAL2 gene in S. cerevisiae and adjacent to the TATA element of the adenovirus major late promoter. A factor (termed USF) that binds to the latter site and stimulates transcription has been isolated from HeLa cells by others.

MeSH Terms
Base Sequence Centromere/metabolism Chromosomes/metabolism DNA-Binding Proteins/genetics,isolation & purification Genes Genes, Fungal Genes, Regulator Humans Molecular Weight Plasmids Saccharomyces cerevisiae/genetics,metabolism Sequence Homology, Nucleic Acid Species Specificity Transcription Factors/genetics
Chemicals
DNA-Binding Proteins Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bram R J
Kornberg R D
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44 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.1.403-409.1987
Published
1987-01-00
Pages
403-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365082
Subset
IM
Grants
NIGMS NIH HHS · GM07365 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.1.403-409.1987 PMC Full Text View journal details
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