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PMID: 6267582 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete nucleotide sequence of minicircle kinetoplast DNA from Trypanosoma equiperdum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 6 ·1981-06-00 ·Pages 3323-7

Barrois M, Riou G, Galibert F

Abstract

The kinetoplast DNA of Trypanosoma equiperdum is composed of about 3000 supercoiled minicircles of 1000 base pairs and about 50 supercoiled maxicircles of 23,000 base pairs topologically interlocked so as to form a compact network. Minicircles of T. equiperdum, which are homogeneous in base sequence, were purified by equilibrium CsCl centrifugation and used as starting material for DNA sequence analysis. One minicircle is composed of 1012 base pairs and has an adenine.thymine base pair content of 72.8%. The termination codons are uniformly distributed along the molecule and restrict the coding potentiality of the molecule to oligopeptides of about 20 amino acids. The molecule contains three dyad symmetries and a sequence of 12 nucleotides is repeated six times. We also noted the presence of a region of about 130 base pairs that is almost perfectly homologous with that of the minicircles from the closely related species T. brucei.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes/metabolism DNA, Circular/genetics DNA, Mitochondrial/genetics Trypanosoma/genetics,ultrastructure
Chemicals
DNA, Circular DNA, Mitochondrial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barrois M
Riou G
Galibert F
References (28)
28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.6.3323
Published
1981-06-00
Pages
3323-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319560
Subset
IM
Databases
GENBANK
J01456
External Links
PubMed source DOI: 10.1073/pnas.78.6.3323 PMC Full Text View journal details
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