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PMID: 2555789 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcriptional activation of cKi-ras proto-oncogene resulting from retroviral promoter insertion.

Nucleic acids research ·Vol. 17 ·No. 22 ·1989-11-25 ·Pages 9259-65

Trusko SP, Hoffman EK, George DL

Abstract

Enhanced expression of the cKi-ras proto-oncogene in a bone marrow-derived mouse cell line, 416B, has been shown to be associated with the integration of Friend viral DNA into the cellular gene. Here we report the results of experiments designed to clarify the molecular mechanism responsible for the cKi-ras overexpression. Based on primer extension analyses and DNA sequencing of cKi-ras cDNA clones, we have obtained evidence that the 416B cells contain viral-host chimaeric transcripts that initiate within the 3' long terminal repeat (LTR) of the integrated provirus. Processing of the transcripts from the rearranged cKi-ras gene includes an unexpected splicing event associated with the fortuitous creation of a cryptic donor splice site at the junction between the proviral and cellular DNA sequences. These data demonstrate that enhanced cKi-ras expression in the 416B cells results from a retroviral promoter insertion mechanism of transcriptional activation.

MeSH Terms

Animals Base Sequence Bone Marrow Cell Line DNA Transposable Elements Exons Female Friend murine leukemia virus/genetics Gene Expression Regulation, Neoplastic Genes, ras Mice Mice, Inbred Strains Molecular Sequence Data Oligonucleotide Probes Promoter Regions, Genetic Proto-Oncogene Mas Transcriptional Activation

Chemicals

DNA Transposable Elements MAS1 protein, human Oligonucleotide Probes Proto-Oncogene Mas

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Trusko S P

Department of Human Genetics, University of Pennsylvania School of Medicine, Philadelphia 19104-6072.

Hoffman E K

George D L

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/17.22.9259

Published

1989-11-25

Pages

9259-65

Language

English

Region

England

NLM ID

0411011

PMCID

PMC335129

Subset

Grants

NCI NIH HHS · CA34462 · United States

NIGMS NIH HHS · GM32592 · United States

Databases

GENBANK

X15887, X15888

External Links

PubMed source DOI: 10.1093/nar/17.22.9259 PMC Full Text View journal details

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Transcriptional activation of cKi-ras proto-oncogene resulting from retroviral promoter insertion.

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