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PMID: 3513183 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Enhanced c-Ki-ras expression associated with Friend virus integration in a bone marrow-derived mouse cell line.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 6 ·1986-03-00 ·Pages 1651-5

George DL, Glick B, Trusko S, Freeman N

Abstract

We have investigated the molecular basis for a 25- to 30-fold overexpression of the c-Ki-ras oncogene in a mouse bone marrow-derived, early myeloid cell line, 416B. Southern blot hybridizations revealed that the 416B cells contain a rearranged c-Ki-ras gene in addition to an apparently normal gene. Molecular cloning and DNA sequence analyses demonstrated that the rearrangement involves the insertion of a 3.5-kilobase-pair segment of Friend virus that includes the envelope gene (env) and 3' long terminal repeat. The Friend provirus is positioned between a 5' nontranslated exon (exon phi) and the first coding exon (exon 1) of the c-Ki-ras gene in the same transcriptional orientation. Results of RNA blot analyses indicate that transcription from the rearranged gene initiates at a promoter that excludes sequences in exon phi. The data support the hypothesis that enhanced c-Ki-ras expression in the 416B cells results from integration of a Friend provirus within this gene.

MeSH Terms
Animals Base Sequence Bone Marrow Cells Cell Line Cell Transformation, Viral Cloning, Molecular Female Friend murine leukemia virus/physiology Gene Expression Regulation Mice Promoter Regions, Genetic Proto-Oncogene Proteins/biosynthesis Proto-Oncogenes RNA, Messenger/analysis Repetitive Sequences, Nucleic Acid Transcription, Genetic Viral Envelope Proteins/genetics
Chemicals
Proto-Oncogene Proteins RNA, Messenger Viral Envelope Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
George D L
Glick B
Trusko S
Freeman N
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.6.1651
Published
1986-03-00
Pages
1651-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323141
Subset
IM
Grants
NCI NIH HHS · CA34462 · United States
NIGMS NIH HHS · GM32592 · United States
Databases
GENBANK
M13293, M13294
External Links
PubMed source DOI: 10.1073/pnas.83.6.1651 PMC Full Text View journal details
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