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PMID: 2555791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The analysis of the human hemopexin promoter defines a new class of liver-specific genes.

Nucleic acids research ·Vol. 17 ·No. 22 ·1989-11-25 ·Pages 9351-65

Poli V, Silengo L, Altruda F, Cortese R

Abstract

Hemopexin (Hpx) is a plasma glycoprotein which is expressed only in the liver. It is synthesized at a lower rate in the fetal liver than in the adult, and its level increases during acute infections. As shown here, a fragment of the human hemopexin promoter spanning from positions -130 to +22 relative to the cap site is sufficient to direct cell-specific transcription of a reporter gene. Within this segment a short sequence, located between positions -120 and -104, is responsible for this effect. This positive cis-acting element, the Hpx A site, interacts with a family of nuclear proteins, some of which are present only in hepatoma cells. The potential meaning of these complex DNA-protein interactions and the homology with elements present on the promoter of other liver-specific and acute phase genes are discussed.

MeSH Terms
Base Sequence Carcinoma, Hepatocellular Cell Line Chromosome Deletion Genes HeLa Cells/metabolism Hemopexin/genetics Humans Liver/metabolism Liver Neoplasms Molecular Sequence Data Mutation Plasmids Promoter Regions, Genetic Templates, Genetic
Chemicals
Hemopexin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Poli V
European Molecular Biology Laboratory, Heidelberg, FRG.
Silengo L
Altruda F
Cortese R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1989-11-25
Pages
9351-65
Language
English
Region
England
NLM ID
0411011
PMCID
PMC335137
Subset
IM
Databases
GENBANK
X15399
External Links
PubMed source PMC Full Text View journal details
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