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PMID: 2558283 Published · ppublish English Journal Article

Molecular characterization of a specific p-nitrophenylphosphatase gene, PHO13, and its mapping by chromosome fragmentation in Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 220 ·No. 1 ·1989-12-00 ·Pages 133-9

Kaneko Y, Toh-e A, Banno I, Oshima Y

Abstract

The structural gene, PHO13, for the specific p-nitrophenyl phosphatase of Saccharomyces cerevisiae was cloned and its nucleotide sequence determined. The deduced PHO13 protein consists of 312 amino acids and its molecular weight is 34635. The disruption of the PHO13 gene produced no effect on cell growth, sporulation, or viability of ascospores. The PHO13 locus was mapped at 1.9 centimorgans from the HO locus on the left arm of chromosome IV. By chromosome fragmentation, the PHO13 locus was found to be located about 72 kb from the left-hand telomere of chromosome IV and distal to the HO locus.

MeSH Terms
4-Nitrophenylphosphatase/genetics Base Sequence Chromosome Banding Chromosomes Cloning, Molecular Genes, Fungal Molecular Sequence Data Phosphoric Monoester Hydrolases/genetics Plasmids Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics,physiology Spores, Fungal/genetics
Chemicals
Phosphoric Monoester Hydrolases 4-Nitrophenylphosphatase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kaneko Y
Institute for Fermentation, Osaka, Japan.
Toh-e A
Banno I
Oshima Y
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00260867
Published
1989-12-00
Pages
133-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00260867
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