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PMID: 3537721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases.

Molecular and cellular biology ·Vol. 6 ·No. 7 ·1986-07-00 ·Pages 2500-10

Woolford CA, Daniels LB, Park FJ, Jones EW, Van Arsdell JN, Innis MA

Abstract

pep4 mutants of Saccharomyces cerevisiae accumulate inactive precursors of vacuolar hydrolases. The PEP4 gene was isolated from a genomic DNA library by complementation of the pep4-3 mutation. Deletion analysis localized the complementing activity to a 1.5-kilobase pair EcoRI-XhoI restriction enzyme fragment. This fragment was used to identify an 1,800-nucleotide mRNA capable of directing the synthesis of a 44,000-dalton polypeptide. Southern blot analysis of yeast genomic DNA showed that the PEP4 gene is unique; however, several related sequences exist in yeasts. Tetrad analysis and mitotic recombination experiments localized the PEP4 gene proximal to GAL4 on chromosome XVI. Analysis of the DNA sequence indicated that PEP4 encodes a polypeptide with extensive homology to the aspartyl protease family. A comparison of the PEP4 predicted amino acid sequence with the yeast protease A protein sequence revealed that the two genes are, in fact, identical (see also Ammerer et al., Mol. Cell. Biol. 6:2490-2499, 1986). Based on our observations, we propose a model whereby inactive precursor molecules produced from the PEP4 gene self-activate within the yeast vacuole and subsequently activate other vacuolar hydrolases.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Deletion Cloning, Molecular Genetic Complementation Test Hydrolases/metabolism Organoids/enzymology Peptide Hydrolases/genetics Protein Processing, Post-Translational Saccharomyces cerevisiae/enzymology,genetics,ultrastructure Transcription, Genetic Vacuoles/enzymology
Chemicals
Hydrolases Peptide Hydrolases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Woolford C A
Daniels L B
Park F J
Jones E W
Van Arsdell J N
Innis M A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.7.2500-2510.1986
Published
1986-07-00
Pages
2500-10
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367804
Subset
IM
Grants
NIADDK NIH HHS · AM18090 · United States
NIGMS NIH HHS · GM09855 · United States
NIGMS NIH HHS · GM29713 · United States
Databases
GENBANK
M13358, M13632
External Links
PubMed source DOI: 10.1128/mcb.6.7.2500-2510.1986 PMC Full Text View journal details
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