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PMID: 2564676 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Formation of pilin in Pseudomonas aeruginosa requires the alternative sigma factor (RpoN) of RNA polymerase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 6 ·1989-03-00 ·Pages 1954-7

Ishimoto KS, Lory S

Abstract

The promoter region of the Pseudomonas aeruginosa pilin gene has a high degree of similarity to the nitrogen-regulated promoters of enteric bacteria. These promoters are recognized by the alternative sigma factor of RNA polymerase, termed RpoN (NtrA or GlnF). This observation suggested that the P. aeruginosa pilin gene may be transcribed by the RpoN-containing RNA polymerase. We, therefore, cloned the RpoN gene from P. aeruginosa into Escherichia coli (where it formed a functional product) and used that cloned gene to construct a mutant of P. aeruginosa that was insertionally inactivated in its RpoN gene. This mutant failed to synthesize pilin, indicating that the RpoN sigma factor is required for transcription of the pilin gene.

MeSH Terms
Bacterial Outer Membrane Proteins/biosynthesis,genetics Cloning, Molecular DNA-Directed RNA Polymerases/genetics Escherichia coli/genetics Fimbriae Proteins Fimbriae, Bacterial Gene Expression Regulation Mutation Nucleic Acid Hybridization Plasmids Promoter Regions, Genetic Pseudomonas aeruginosa/genetics Sigma Factor/genetics Transcription Factors/genetics Transcription, Genetic Transformation, Bacterial beta-Galactosidase/genetics,metabolism
Chemicals
Bacterial Outer Membrane Proteins Sigma Factor Transcription Factors Fimbriae Proteins DNA-Directed RNA Polymerases beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ishimoto K S
Department of Microbiology, School of Medicine, University of Washington, Seattle 98195.
Lory S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.6.1954
Published
1989-03-00
Pages
1954-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286823
Subset
IM
Grants
NIGMS NIH HHS · 5 T32 GMO7270 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.6.1954 PMC Full Text View journal details
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