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PMID: 25693803 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Discovery of predictive biomarkers for litter size in boar spermatozoa.

Molecular & cellular proteomics : MCP ·Vol. 14 ·No. 5 ·2015-05-00 ·Pages 1230-40

Kwon WS, Rahman MS, Lee JS, Yoon SJ, Park YJ, Pang MG

Abstract

Conventional semen analysis has been used for prognosis and diagnosis of male fertility. Although this tool is essential for providing initial quantitative information about semen, it remains a subject of debate. Therefore, development of new methods for the prognosis and diagnosis of male fertility should be seriously considered for animal species of economic importance as well as for humans. In the present study, we applied a comprehensive proteomic approach to identify global protein biomarkers in boar spermatozoa in order to increase the precision of male fertility prognoses and diagnoses. We determined that l-amino acid oxidase, mitochondrial malate dehydrogenase 2, NAD (MDH2), cytosolic 5'-nucleotidase 1B, lysozyme-like protein 4, and calmodulin (CALM) were significantly and abundantly expressed in high-litter size spermatozoa. We also found that equatorin, spermadhesin AWN, triosephosphate isomerase (TPI), Ras-related protein Rab-2A (RAB2A), spermadhesin AQN-3, and NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 (NDUFS2) were significantly and abundantly expressed in low-litter size spermatozoa (>3-fold). Moreover, RAB2A, TPI, and NDUFS2 were negatively correlated with litter size, whereas CALM and MDH2 were positively correlated. This study provides novel biomarkers for the prediction of male fertility. To the best of our knowledge, this is the first work that shows significantly increased litter size using male fertility biomarkers in a field trial. Moreover, these protein markers may provide new developmental tools for the selection of superior sires as well as for the prognosis and diagnosis of male fertility.

MeSH Terms
Amino Acid Sequence Animals Biomarkers/metabolism Female Fertility/genetics Gene Expression Litter Size/genetics Malate Dehydrogenase (NADP+)/genetics,metabolism Male Molecular Sequence Annotation Molecular Sequence Data Monomeric Clathrin Assembly Proteins/genetics,metabolism NADH Dehydrogenase/genetics,metabolism Predictive Value of Tests Protein Interaction Mapping Semen Analysis/methods Spermatozoa/chemistry,enzymology Swine Triose-Phosphate Isomerase/genetics,metabolism rab GTP-Binding Proteins/genetics,metabolism
Chemicals
Biomarkers Monomeric Clathrin Assembly Proteins Malate Dehydrogenase (NADP+) NADH Dehydrogenase rab GTP-Binding Proteins Triose-Phosphate Isomerase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kwon Woo-Sung
From the ‡Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Republic of Korea.
Rahman Md Saidur
From the ‡Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Republic of Korea.
Lee June-Sub
From the ‡Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Republic of Korea.
Yoon Sung-Jae
From the ‡Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Republic of Korea.
Park Yoo-Jin
From the ‡Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Republic of Korea.
Pang Myung-Geol
From the ‡Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-do 456-756, Republic of Korea. [email protected].
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Article Info
Journal
Molecular & cellular proteomics : MCP
Abbr.
Mol Cell Proteomics
ISSN
1535-9484
DOI
10.1074/mcp.M114.045369
Email
[email protected]
Published
2015-05-00
Epub
2015-00-18
Pages
1230-40
Language
English
Region
United States
NLM ID
101125647
PMCID
PMC4424395
Subset
IM
External Links
PubMed source DOI: 10.1074/mcp.M114.045369 PMC Full Text View journal details
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