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PMID: 2573513 Published · ppublish English Journal Article

Human herpes virus-6 increases HIV-1 expression in co-infected T cells via nuclear factors binding to the HIV-1 enhancer.

The EMBO journal ·Vol. 8 ·No. 10 ·1989-10-00 ·Pages 3019-27

Ensoli B, Lusso P, Schachter F, Josephs SF, Rappaport J, Negro F, Gallo RC, Wong-Staal F

Abstract

Human Herpes virus-6 (HHV-6) can co-infect with HIV-1 human CD4+ T-cells, leading to accelerated cell death, and factors in HHV-6-infected cells stimulate HIV-1 LTR directed gene expression. In this study, we have examined the mechanism of HIV-1 activation by HHV-6 and localized the cis-acting sequences of HIV-1 LTR responsive to trans-activation. Increased HIV-1 LTR directed gene expression is obtained in HIV-1 infected cells co-infected with HHV-6, or in HHV-6 infected cells co-transfected with the HIV-1 tat gene. Parallel increases of HIV-1-specific transcripts are seen by in situ hybridization in HHV-6/HIV-1 doubly infected cells as compared to single HIV-1 infection. Similarly, infection by HHV-6 increases the steady-state level of HIV-1 LTR mRNA that parallels CAT enzymatic activity, suggesting a transcriptional and/or post-transcriptional activation. Sequences necessary for HIV-1 LTR activation by HHV-6 are distinct from those required for that tat response and map to a region of the HIV-1 LTR from -103 to -48. The HIV-1 enhancer sequence (-105 to -80) is sufficient to confer HHV-6 inducibility to a heterologous promoter, and nuclear protein(s) activated or induced by HHV-6 infection specifically bind to the NF kappa B motifs of the HIV-1 enhancer region.(ABSTRACT TRUNCATED AT 250 WORDS)

MeSH Terms
Base Sequence CD4-Positive T-Lymphocytes/microbiology Cell Line DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Gene Expression Regulation, Viral Genes, Viral HIV-1/genetics,physiology Herpesvirus 6, Human/genetics,physiology Humans Mutation Nuclear Proteins/metabolism Nucleic Acid Hybridization Promoter Regions, Genetic RNA, Messenger/genetics RNA, Viral/genetics Transcription, Genetic Transcriptional Activation Transfection Virus Activation
Chemicals
DNA-Binding Proteins Nuclear Proteins RNA, Messenger RNA, Viral
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ensoli B
Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20892.
Lusso P
Schachter F
Josephs S F
Rappaport J
Negro F
Gallo R C
Wong-Staal F
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-10-00
Pages
3019-27
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401379
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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