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PMID: 6098465 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Stimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans-acting factor.

The EMBO journal ·Vol. 3 ·No. 13 ·1984-12-20 ·Pages 3129-33

Wildeman AG, Sassone-Corsi P, Grundström T, Zenke M, Chambon P

Abstract

A nuclear extract prepared from HeLa cells has been used to study in vitro the transcription of the SV40 early promoter. The deletion of the enhancer results in a strong decrease of transcription, with spermidine and MgCl2 being critical variables in the transcription reactions. Furthermore a competition assay indicates that the stimulation by the enhancer is due to a specific trans-acting factor(s) which acts on it. This factor appears not to interact with SV40 or adenovirus-2 major late upstream (distal) promoter sequences, and its ability to bind to the enhancer is diminished by mutations known to decrease enhancer function in vivo and in vitro.

MeSH Terms
Enhancer Elements, Genetic Genes, Regulator HeLa Cells/metabolism Humans In Vitro Techniques Magnesium/pharmacology Magnesium Chloride Mutation Operon Simian virus 40/genetics Spermidine/pharmacology Transcription, Genetic/drug effects
Chemicals
Magnesium Chloride Magnesium Spermidine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wildeman A G
Sassone-Corsi P
Grundström T
Zenke M
Chambon P
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29 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-12-20
Pages
3129-33
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557828
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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