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PMID: 2573821 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In organello replication and viral affinity of linear, extrachromosomal DNA of the ascomycete Ascobolus immersus.

Molecular & general genetics : MGG ·Vol. 218 ·No. 3 ·1989-09-00 ·Pages 523-30

Kempken F, Meinhardt F, Esser K

Abstract

Linear, extrachromosomal DNA's of the filamentous fungus Ascobolus immersus are localized within the mitochondria. These linear plasmids have no homology to the high molecular weight mtDNA (hmw mtDNA). For analysis of plasmid replication an in organello DNA synthesis system was developed, in which radionucleotides were incorporated into intact mitochondria. Plasmid DNA is labelled preferentially in this system. From replication analysis of a specific plasmid there is evidence of a virus-like protein-primed replication. Sequence analysis of this plasmid reveals that a viral DNA polymerase is encoded. Thus, these genetic elements presumably are viral remnants rather than true plasmids.

MeSH Terms
Amino Acid Sequence Ascomycota/genetics Base Sequence Codon DNA Replication DNA, Mitochondrial/isolation & purification DNA-Directed DNA Polymerase/metabolism Extrachromosomal Inheritance Fungal Proteins/genetics Molecular Sequence Data Nucleic Acid Hybridization Plasmids Polymorphism, Restriction Fragment Length Sequence Homology, Nucleic Acid Viral Proteins/genetics
Chemicals
Codon DNA, Mitochondrial Fungal Proteins Viral Proteins DNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kempken F
Ruhr-Universität Bochum, Lehrstuhl für Allgemeine Botanik, Federal Republic of Germany.
Meinhardt F
Esser K
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332419
Published
1989-09-00
Pages
523-30
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X15982
External Links
PubMed source DOI: 10.1007/BF00332419
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