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PMID: 6387625 Published · ppublish English Journal Article

Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast.

Nucleic acids research ·Vol. 12 ·No. 19 ·1984-10-11 ·Pages 7581-97

Hishinuma F, Nakamura K, Hirai K, Nishizawa R, Gunge N, Maeda T

Abstract

The linear DNA killer plasmids (pGKL1 and pGKL2) isolated from a Kluyveromyces lactis killer strain are also maintained and expressed its killer character in Saccharomyces cerevisiae. After these killer plasmid DNAs isolated from S. cerevisiae were treated with alkali, four terminal fragments from each plasmid DNAs were cloned separately. Using these and other cloned DNA fragments, the terminal nucleotide sequences of pGKL2 and the complete nucleotide sequence of pGKL1 were determined. The inverted terminal repetitions of 202 bp and 182 bp were found in pGKL1 and pGKL2, respectively. The pGKL1 sequence showed an extremely high A + T content of 73.2% and it contained five large open reading frames. The largest of these open reading frame was suggested to code for a membrane-bound precursor of glycoprotein subunit of the killer toxin.

MeSH Terms
Base Sequence Cloning, Molecular Escherichia coli/genetics Plasmids Saccharomyces cerevisiae/genetics Yeasts/genetics
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hishinuma F
Nakamura K
Hirai K
Nishizawa R
Gunge N
Maeda T
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43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.19.7581
Published
1984-10-11
Pages
7581-97
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320182
Subset
IM
Databases
GENBANK
M11815, X01096, X01097
External Links
PubMed source DOI: 10.1093/nar/12.19.7581 PMC Full Text View journal details
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