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PMID: 2580230 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Increased phosphorylation of tyrosine in vinculin does not occur upon transformation by some avian sarcoma viruses.

Molecular and cellular biology ·Vol. 5 ·No. 1 ·1985-01-00 ·Pages 263-7

Antler AM, Greenberg ME, Edelman GM, Hanafusa H

Abstract

The level of phosphotyrosine in vinculin was determined in chicken embryo fibroblasts transformed by various strains of avian sarcoma virus. As previously reported (Sefton et al., Cell 24:165-174, 1981), vinculin was phosphorylated at tyrosine residues in most cultures examined, but the level varied greatly and no detectable change was found in cultures infected with Fujinami sarcoma virus or UR2 sarcoma virus. Regardless of the level of vinculin phosphorylation, the number of organized microfilament bundles was found to be decreased in all transformed cells. These results strongly suggest that tyrosine phosphorylation of vinculin is not an obligatory step in cell transformation by this class of oncogenes, nor is it correlated with the associated cytoskeletal disarray.

MeSH Terms
Animals Avian Sarcoma Viruses Cell Transformation, Viral Cells, Cultured Chick Embryo Cytoskeleton/ultrastructure Muscle Proteins/metabolism Phosphorylation Phosphotyrosine Tyrosine/analogs & derivatives,metabolism Vinculin
Chemicals
Muscle Proteins Vinculin Phosphotyrosine Tyrosine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Antler A M
Greenberg M E
Edelman G M
Hanafusa H
References (33)
33 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.1.263-267.1985
Published
1985-01-00
Pages
263-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366703
Subset
IM
Grants
NIAID NIH HHS · AI11378 · United States
NCI NIH HHS · CA14935 · United States
NCI NIH HHS · CA18213 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.1.263-267.1985 PMC Full Text View journal details
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