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PMID: 6289331 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of 16L virus: a rapidly transforming sarcoma virus from an avian leukosis virus-induced sarcoma.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 16 ·1982-08-00 ·Pages 5088-92

Neel BG, Wang LH, Mathey-Prevot B, Hanafusa T, Hanafusa H, Hayward WS

Abstract

We have isolated a replication-defective rapidly transforming sarcoma virus (designated 16L virus) from a fibro-sarcoma in a chicken infected with td107A, a transformation-defective deletion mutant of subgroup A Schmidt-Ruppin Rous sarcoma virus. 16L virus transforms fibroblasts and causes sarcomas in infected chickens within 2 wk. Its genomic RNA is 6.0 kilobases and contains sequences homologous to the transforming gene (fps) of Fujinami sarcoma virus (FSV). RNase T1 oligonucleotide analysis shows that the 5' and 3' terminal sequences of 16L virus are indistinguishable from (and presumably derived from) td107A RNA. The central part of 16L viral RNA consists of fps-related sequences. These oligonucleotides fall into four classes: (i) oligonucleotides common to the putative transforming regions of FSV and another fps-containing avian sarcoma virus, UR1; (ii) an oligonucleotide also present in FSV but not in UR1; (iii) an oligonucleotide also present in UR1 but not in FSV; and (iv) an oligonucleotide not present in either FSV, UR1, or td107A. Cells infected with 16L virus synthesize a protein of Mr 142,000 that is immunoprecipitated with anti-gag antiserum. This protein has protein kinase activity. These results suggest that 16L virus arose by recombination between td107A and the cellular fps gene.

MeSH Terms
Animals Avian Sarcoma Viruses/isolation & purification Cells, Cultured Chick Embryo Molecular Weight Oligoribonucleotides/analysis RNA, Viral/analysis Sarcoma, Experimental/microbiology Viral Proteins/analysis
Chemicals
Oligoribonucleotides RNA, Viral Viral Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Neel B G
Wang L H
Mathey-Prevot B
Hanafusa T
Hanafusa H
Hayward W S
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47 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.16.5088
Published
1982-08-00
Pages
5088-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346833
Subset
IM
Grants
NCI NIH HHS · CA 14935 · United States
NCI NIH HHS · CA 16668 · United States
NCI NIH HHS · CA 29339 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.16.5088 PMC Full Text View journal details
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