Home LiteratureArticle Details
PMID: 2582364 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Association of the lupus antigen La with a subset of U6 snRNA molecules.

Nucleic acids research ·Vol. 13 ·No. 7 ·1985-04-11 ·Pages 2617-29

Rinke J, Steitz JA

Abstract

U6 snRNA is a component of the major class of small RNA-protein complexes, the Sm snRNPs, present in mammalian cell nuclei. Here we report that a substantial fraction (about 10%) of U6 RNA from human and mouse cells is associated with another lupus antigen, the 50 kd La protein. The La-bound U6 subpopulation is characterized by 3' end heterogeneity and partial undermethylation. These U6 molecules have U-rich 3' termini that could be responsible for their selective association with the La protein. The question of whether they are precursors to the major U6 RNAs found in Sm snRNPs is discussed.

MeSH Terms
Animals Antigens/analysis Autoantigens/analysis Base Sequence Carcinoma, Ehrlich Tumor/analysis DNA-Directed RNA Polymerases/metabolism HeLa Cells/analysis Humans Mice Nucleic Acid Hybridization RNA/analysis RNA, Small Nuclear Ribonucleoproteins
Chemicals
Antigens Autoantigens RNA, Small Nuclear Ribonucleoproteins SS-B antigen RNA DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rinke J
Steitz J A
References (47)
47 references, click to expand
  1. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  2. Human U1 RNA pseudogenes may be generated by both DNA- and RNA-mediated mechanisms.
    Mol Cell Biol. 1982 Jul;2(7):815-28 PMID: 6085956
  3. Low molecular weight RNAs hydrogen-bonded to nuclear and cytoplasmic poly(A)-terminated RNA from cultured Chinese hamster ovary cells.
    Cell. 1978 Sep;15(1):205-14 PMID: 699042
  4. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  5. Subsets in systemic lupus erythematosus.
    J Invest Dermatol. 1979 Mar;72(3):110-3 PMID: 370316
  6. Synthesis of low molecular weight RNA components A, C and D by polymerase II in alpha-amanitin-resistant hamster cells.
    Nucleic Acids Res. 1979 Jan;6(1):321-30 PMID: 424295
  7. Resolution of the identity of certain antigen-antibody systems in systemic lupus erythematosus and Sjögren's syndrome: an interlaboratory collaboration.
    Arthritis Rheum. 1979 Jul;22(7):796-8 PMID: 110340
  8. Series of 4.5S RNAs associated with poly(A)-containing RNAs of rodent cells.
    Nucleic Acids Res. 1979 Oct 25;7(4):909-17 PMID: 116194
  9. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5495-9 PMID: 316537
  10. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
    Methods Enzymol. 1979;68:220-42 PMID: 94421
  11. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  12. Ubiquitous, interspersed repeated sequences in mammalian genomes.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1398-402 PMID: 6246492
  13. The nucleotide sequence of nuclear U6 (4.7 S) RNA.
    J Biol Chem. 1980 Sep 25;255(18):8901-6 PMID: 6773955
  14. The nucleotide sequence of nuclear 4.8S RNA of mouse cells.
    Biochem Biophys Res Commun. 1980 Aug 14;95(3):1332-40 PMID: 6251836
  15. Nucleotide sequences of 4.5S RNAs associated with poly(A)-containing RNAs of mouse and hamster cells.
    Nucleic Acids Res. 1980 Mar 25;8(6):1273-85 PMID: 6159592
  16. Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus.
    Science. 1981 Jan 23;211(4480):400-2 PMID: 6164096
  17. Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):805-9 PMID: 6262773
  18. Abundant pseudogenes for small nuclear RNAs are dispersed in the human genome.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):810-4 PMID: 6165010
  19. Structural analysis of templates and RNA polymerase III transcripts of Alu family sequences interspersed among the human beta-like globin genes.
    Gene. 1981 Mar;13(2):185-96 PMID: 6165649
  20. Organization of sequences related to U6 RNA in the human genome.
    Nucleic Acids Res. 1981 Jul 24;9(14):3379-88 PMID: 6169006
  21. Transcriptional control regions: nucleotide sequence requirements for initiation by RNA polymerase II and III.
    Curr Top Microbiol Immunol. 1981;93:25-46 PMID: 7026181
  22. Nucleotide sequences of mouse genomic loci including a gene or pseudogene for U6 (4.8S) nuclear RNA.
    Nucleic Acids Res. 1981 Oct 10;9(19):5145-58 PMID: 6171774
  23. Low molecular weight RNAs transcribed in vitro by RNA polymerase III from Alu-type dispersed repeats in Chinese hamster DNA are also found in vivo.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6130-4 PMID: 6796957
  24. Precursor molecules of both human 5S ribosomal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La lupus antibodies.
    Cell. 1982 May;29(1):149-59 PMID: 7105180
  25. Human 7SL RNA consists of a 140 nucleotide middle-repetitive sequence inserted in an alu sequence.
    Cell. 1982 May;29(1):195-202 PMID: 6179628
  26. The organization, structure, and in vitro transcription of Alu family RNA polymerase III transcription units in the human alpha-like globin gene cluster: precipitation of in vitro transcripts by lupus anti-La antibodies.
    J Mol Appl Genet. 1982;1(4):343-60 PMID: 6286832
  27. Striking similarities are exhibited by two small Epstein-Barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids VAI and VAII.
    Mol Cell Biol. 1981 Sep;1(9):785-96 PMID: 9279391
  28. Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.
    Mol Cell Biol. 1981 Dec;1(12):1138-49 PMID: 6180298
  29. Interaction between VA RNA and the lupus antigen La: formation of a ribonucleoprotein particle in vitro.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6772-6 PMID: 6184716
  30. Sequential association of nucleolar 7-2 RNA with two different autoantigens.
    J Biol Chem. 1983 Feb 10;258(3):1379-82 PMID: 6185483
  31. Detection of a nucleolar 7-2 ribonucleoprotein and a cytoplasmic 8-2 ribonucleoprotein with autoantibodies from patients with scleroderma.
    J Biol Chem. 1983 Feb 10;258(3):1383-6 PMID: 6185484
  32. Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome.
    Cell. 1983 Mar;32(3):735-44 PMID: 6187471
  33. Structural organization of ribonucleoproteins containing small nuclear RNAs from HeLa cells. Proteins interact closely with a similar structural domain of U1, U2, U4 and U5 small nuclear RNAs.
    J Mol Biol. 1982 Dec 15;162(3):623-43 PMID: 6187926
  34. Small ribonucleoproteins from eukaryotes: structures and roles in RNA biogenesis.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:893-900 PMID: 6222869
  35. Identification of a La protein binding site in a RNA polymerase III transcript (4.5 I RNA).
    J Biol Chem. 1983 Jul 10;258(13):8352-6 PMID: 6863291
  36. Association between the 7 S RNA and the lupus La protein varies among cell types.
    J Biol Chem. 1983 Oct 10;258(19):11438-41 PMID: 6194154
  37. The leader RNA of vesicular stomatitis virus is bound by a cellular protein reactive with anti-La lupus antibodies.
    Cell. 1983 Oct;34(3):837-45 PMID: 6313210
  38. Characterization of the SS-B (La) antigen in adenovirus-infected and uninfected HeLa cells.
    EMBO J. 1983;2(10):1625-31 PMID: 6357777
  39. Purified lupus antigen La recognizes an oligouridylate stretch common to the 3' termini of RNA polymerase III transcripts.
    Cell. 1984 Jan;36(1):145-54 PMID: 6607117
  40. Eukaryotic small ribonucleoproteins. Anti-La human autoantibodies react with U1 RNA-protein complexes.
    J Biol Chem. 1984 Feb 10;259(3):1929-33 PMID: 6229541
  41. Transcription in vivo of an Alu family member upstream from the human epsilon-globin gene.
    Nucleic Acids Res. 1984 Jan 25;12(2):1193-200 PMID: 6320117
  42. Small nuclear RNAs and RNA processing.
    Prog Nucleic Acid Res Mol Biol. 1983;30:127-62 PMID: 6198692
  43. U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle.
    Nucleic Acids Res. 1984 Apr 11;12(7):3283-93 PMID: 6201826
  44. U2 RNA shares a structural domain with U1, U4, and U5 RNAs.
    EMBO J. 1982;1(10):1259-65 PMID: 6202507
  45. La antigen recognizes and binds to the 3'-oligouridylate tail of a small RNA.
    Mol Cell Biol. 1984 Jun;4(6):1134-40 PMID: 6738534
  46. Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing.
    EMBO J. 1984 Jun;3(6):1357-63 PMID: 6204860
  47. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.7.2617
Published
1985-04-11
Pages
2617-29
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341179
Subset
IM
Grants
NCI NIH HHS · CA16038 · United States
NIGMS NIH HHS · GM26154 · United States
External Links
PubMed source DOI: 10.1093/nar/13.7.2617 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2