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PMID: 424295 Published · ppublish English Journal Article

Synthesis of low molecular weight RNA components A, C and D by polymerase II in alpha-amanitin-resistant hamster cells.

Nucleic acids research ·Vol. 6 ·No. 1 ·1979-01-00 ·Pages 321-30

Jensen EG, Hellung-Larsen P, Frederiksen S

Abstract

In an attempt to establish which RNA polymerase catalyzes the synthesis of the low molecular weight RNA components A, C and D, Ama 1 cells (mutant Chinese hamster cells) were used in experiments with addition of alpha-amanitin. Ama 1 cells contain an altered RNA polymerase II which is 800 times more resistant towards inhibition by alpha-amanitin than the wild type enzyme. Alpha-amanitin (up to 200 microgram/ml) added to these cells does not affect the synthesis of the low molecular weight RNAs A, C and D. These data together with our previous data showing that alpha-amanitin (0.5 - 5.0 microgram/ml) preferentially inhibits the synthesis of A, C and D in normal cells indicate that RNA polymerase II catalyzes the synthesis of the low molecular weight RNA components A, C and D.

MeSH Terms
Amanitins/pharmacology Cell Line DNA-Directed RNA Polymerases/metabolism Drug Resistance Molecular Weight RNA/biosynthesis RNA Polymerase II/metabolism
Chemicals
Amanitins RNA RNA Polymerase II DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jensen E G
Hellung-Larsen P
Frederiksen S
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.1.321
Published
1979-01-00
Pages
321-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327691
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.1.321 PMC Full Text View journal details
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