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PMID: 25825237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A proteomic analysis on human sperm tail: comparison between normozoospermia and asthenozoospermia.

Journal of assisted reproduction and genetics ·Vol. 32 ·No. 6 ·2015-06-00 ·Pages 853-63

Hashemitabar M, Sabbagh S, Orazizadeh M, Ghadiri A, Bahmanzadeh M

Abstract

Asthenozoospermia is a common cause of human male infertility characterized by reduced sperm motility. The molecular mechanism that impairs sperm motility is not fully understood. This study proposed to identify novel biomarkers by focusing on sperm tail proteomic analysis of asthenozoospermic patients. Sperm were isolated from normozoospermic and asthenozoospermic semen samples. Tail fractions were obtained by sonication followed by Percoll gradient. The proteins were extracted by solubilization and subjected to two-dimensional gel electrophoresis (2-DE); then, the spots were analyzed using Image Master 2D Platinum software. The significantly increased/decreased amounts of proteins in the two groups were exploited by matrix-assisted laser desorption-ionization time-of-flight/time-of-flight (MALDI-TOF-TOF) mass spectrometry. Three hundred ninety protein spots were detected in both groups. Twenty-one protein spots that had significantly altered amounts (p < 0.05) were excised and exploited using MALDI-TOF-TOF mass spectrometry. They led to the identification of the following 14 unique proteins: Tubulin beta 2B; glutathione S-transferase Mu 3; keratin, type II cytoskeletal 1; outer dense fiber protein 2; voltage-dependent anion-selective channel protein 2; A-kinase anchor protein 4; cytochrome c oxidase subunit 6B; sperm protein associated with the nucleus on the X chromosome B; phospholipid hydroperoxide glutathione peroxidase-mitochondrial; isoaspartyl peptidase/L-asparaginase; heat shock-related 70 kDa protein 2; stress-70 protein, mitochondrial; glyceraldehyde-3-phosphate dehydrogenase, testis-specific and clusterin. Fourteen proteins present in different amounts in asthenozoospermic sperm tail samples were identified, four of which are reported here for the first time. These proteins might be used as markers for the better diagnosis of sperm dysfunctions, targets for male contraceptive development, and to predict embryo quality.

MeSH Terms
Adult Asthenozoospermia/metabolism Biomarkers/metabolism Cell Fractionation Humans Male Proteins/chemistry,isolation & purification,metabolism Proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sperm Motility Sperm Tail/metabolism
Chemicals
Biomarkers Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hashemitabar Mahmoud
Cellular and Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Sabbagh Susan
Orazizadeh Mahmoud
Ghadiri Atta
Bahmanzadeh Maryam
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Article Info
Journal
Journal of assisted reproduction and genetics
Abbr.
J Assist Reprod Genet
ISSN
1573-7330
DOI
10.1007/s10815-015-0465-7
Published
2015-06-00
Epub
2015-00-01
Pages
853-63
Language
English
Region
Netherlands
NLM ID
9206495
PMCID
PMC4491089
Subset
IM
External Links
PubMed source DOI: 10.1007/s10815-015-0465-7 PMC Full Text View journal details
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