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PMID: 2583123 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A thyroid-specific nuclear protein essential for tissue-specific expression of the thyroglobulin promoter.

The EMBO journal ·Vol. 8 ·No. 9 ·1989-09-00 ·Pages 2537-42

Civitareale D, Lonigro R, Sinclair AJ, Di Lauro R

Abstract

A rat thyroglobulin promoter fragment, capable of directing thyroid-specific transcription, binds at least three different factors, TTF-1, TTF-2 and UFA, which are all present in nuclear extracts of the differentiated rat thyroid cell line FRTL-5. TTF-1 and TTF-2 are FRTL-5 specific, as demonstrated by their absence in nuclear extracts prepared from cell lines that do not express any thyroid-differentiated function, while UFA is present in all cell lines tested. TTF-1 has been extensively purified. It binds to the rat thyroglobulin promoter at three different sites which share sequence homology. Mutations in two of the three sites decrease both binding of TTF-1 in vitro and promoter function in vivo. This suggests that the tissue-specific expression of the thyroglobulin genes is mediated, at least in part, by the presence of a transcription factor exclusively in thyroid cells.

MeSH Terms
Animals Base Sequence Binding Sites Cell Line DNA Mutational Analysis DNA-Binding Proteins/isolation & purification,physiology Gene Expression Regulation/physiology Molecular Sequence Data Nuclear Proteins/isolation & purification,physiology Organ Specificity Promoter Regions, Genetic Rats Thyroglobulin/genetics Thyroid Gland/analysis,cytology
Chemicals
DNA-Binding Proteins Nuclear Proteins Thyroglobulin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Civitareale D
European Molecular Biology Laboratory (EMBL), Heidelberg, FRG.
Lonigro R
Sinclair A J
Di Lauro R
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32 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-09-00
Pages
2537-42
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401250
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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