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PMID: 25882 Published · ppublish English Journal Article

Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli.

Journal of bacteriology ·Vol. 134 ·No. 1 ·1978-04-00 ·Pages 147-56

Lopilato J, Tsuchiya T, Wilson TH

Abstract

Thiomethyl-beta-galactoside (TMG) accumulation via the melibiose transport system was studied in lactose transport-negative strains of Escherichia coli. TMG uptake by either intact cells or membrane vesicles was markedly stimulated by Na+ or Li+ between pH 5.5 and 8. The Km for uptake of TMG was approximately 0.2 mM at an external Na+ concentration of 5 mM (pH 7). The alpha-galactosides, melibiose, methyl-alpha-galactoside, and o-nitrophenyl-alpha-galactoside had a high affinity for this system whereas lactose, maltose and glucose had none. Evidence is presented for Li+-TMG or Na+-TMG cotransport.

MeSH Terms
Biological Transport, Active/drug effects Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Disaccharides/metabolism Escherichia coli/metabolism Galactosides/metabolism Glycosides/metabolism Hydrogen-Ion Concentration Lithium/metabolism,pharmacology Membrane Potentials Sodium/metabolism,pharmacology Sulfhydryl Reagents/pharmacology
Chemicals
Disaccharides Galactosides Glycosides Sulfhydryl Reagents Carbonyl Cyanide m-Chlorophenyl Hydrazone Lithium Sodium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lopilato J
Tsuchiya T
Wilson T H
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.134.1.147-156.1978
Published
1978-04-00
Pages
147-56
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222229
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.134.1.147-156.1978 PMC Full Text View journal details
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