Home LiteratureArticle Details
PMID: 2590171 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular beta-lactamase of Actinomadura R39.

The Biochemical journal ·Vol. 262 ·No. 3 ·1989-09-15 ·Pages 849-54

Piron-Fraipont C, Duez C, Matagne A, Molitor C, Dusart J, Frère JM, Ghuysen JM

Abstract

By using the promoter-probe plasmid pIJ424, genomic DNA fragments of Actinomadura R39 were shown to have promoter activity in Streptomyces lividans. The same 100-200-copy-number plasmid was used to clone in S. lividans TK24, the gene that encodes the Actinomadura R39 beta-lactamase. Gene cloning resulted in an amplified expression of the beta-lactamase when compared with the amounts of enzyme produced by the original strain (1 mg versus 0.008 mg.litre of culture-1).

MeSH Terms
Actinomycetales/enzymology Amino Acids/analysis Cloning, Molecular DNA, Bacterial Gene Amplification Kinetics Plasmids Promoter Regions, Genetic Recombinant Proteins/biosynthesis Streptomyces/enzymology,genetics beta-Lactamases/biosynthesis
Chemicals
Amino Acids DNA, Bacterial Recombinant Proteins beta-Lactamases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Piron-Fraipont C
Département de Microbiologie, Université de Liège, Sart Tilman, Belgium.
Duez C
Matagne A
Molitor C
Dusart J
Frère J M
Ghuysen J M
References (26)
26 references, click to expand
  1. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.
    Antimicrob Agents Chemother. 1972 Apr;1(4):283-8 PMID: 4208895
  2. Effects of donor and acceptor peptides on concomitant hydrolysis and transfer reactions catalyzed by the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R39.
    Biochemistry. 1974 Jun 4;13(12):2539-47 PMID: 4831903
  3. The exocellular DD-carboxypeptidase-endopeptidase from Streptomyces albus G. Purification and chemical properties.
    Biochem J. 1978 Dec 1;175(3):793-800 PMID: 743235
  4. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  5. Enzymatic method for rapid and sensitive determination of beta-lactam antibiotics.
    Antimicrob Agents Chemother. 1980 Oct;18(4):506-10 PMID: 7192533
  6. The penicillin-binding site in the exocellular DD-carboxypeptidase-transpeptidase of Actinomadura R39.
    Biochem J. 1981 Jan 1;193(1):83-6 PMID: 7305936
  7. Studies on the primary structures of the exocellular D-alanyl-D-alanine peptidases of Streptomyces strain R61 and Actinomadura strain R39.
    Biochim Biophys Acta. 1981 Dec 29;671(2):109-16 PMID: 7034781
  8. Purification and properties of the exocellular beta-lactamase of Actinomadura strain R39.
    Biochim Biophys Acta. 1982 Jan 4;700(1):24-32 PMID: 6976797
  9. pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors.
    Mol Gen Genet. 1982;185(2):223-8 PMID: 6283316
  10. The complete amino acid sequence of the Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of streptomyces albus G.
    Eur J Biochem. 1983 Jan 17;130(1):53-69 PMID: 6825689
  11. Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.
    J Gen Microbiol. 1983 Jul;129(7):2257-69 PMID: 6631413
  12. Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.
    J Gen Microbiol. 1983 Sep;129(9):2703-14 PMID: 6313861
  13. Purification of beta-lactamases by affinity chromatography on phenylboronic acid-agarose.
    Biochem J. 1984 Jul 15;221(2):505-12 PMID: 6332621
  14. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli.
    Plasmid. 1984 Jul;12(1):19-36 PMID: 6387733
  15. Cloning and amplified expression in Streptomyces lividans of a gene encoding extracellular beta-lactamase from Streptomyces albus G.
    Gene. 1986;42(1):31-6 PMID: 3013728
  16. Construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator.
    Mol Gen Genet. 1986 Jun;203(3):468-78 PMID: 3018431
  17. Primary structure of the Streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces lividans and nucleotide sequence of the gene.
    Eur J Biochem. 1987 Feb 2;162(3):509-18 PMID: 3830154
  18. Automated analysis of enzyme inactivation phenomena. Application to beta-lactamases and DD-peptidases.
    Biochem Pharmacol. 1987 Jul 15;36(14):2393-403 PMID: 3038122
  19. Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular beta-lactamase from Streptomyces cacaoi.
    J Gen Microbiol. 1987 Oct;133(10):2915-20 PMID: 3502415
  20. Transduction of linked genetic characters of the host by bacteriophage P1.
    Virology. 1955 Jul;1(2):190-206 PMID: 13267987
  21. Penicillin-sensitive DD-carboxypeptidase from Streptomyces strain R 61.
    Biochemistry. 1971 May 25;10(11):2163-70 PMID: 4998135
  22. Penicillin-sensitive DD-carboxypeptidases from Streptomyces strains R39 and K11.
    Biochemistry. 1972 Mar 28;11(7):1290-8 PMID: 5012980
  23. Structure of the wall peptidoglycan of Streptomyces R39 and the specificity profile of its exocellular DD-carboxypeptidase--transpeptidase for peptide acceptors.
    Biochemistry. 1973 Mar 27;12(7):1243-51 PMID: 4696752
  24. Maturation of the head of bacteriophage T4. I. DNA packaging events.
    J Mol Biol. 1973 Nov 15;80(4):575-99 PMID: 4204102
  25. Exocellular beta-lactamases of Streptomyces albus G and strains R39 and K11.
    Antimicrob Agents Chemother. 1973 Feb;3(2):289-98 PMID: 4494518
  26. Binding of beta-lactam antibiotics to the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.
    Biochem J. 1974 Oct;143(1):241-9 PMID: 4464853
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2620849
Published
1989-09-15
Pages
849-54
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1133351
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2620849 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2