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PMID: 2594753 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 23 ·1989-12-00 ·Pages 9119-23

Laban A, Wirth DF

Abstract

We report a transient expression transfection system in Leishmania enriettii. A hybrid gene containing an intergenic region of the alpha-tubulin cluster and the bacterial chloramphenicol acetyltransferase (CAT; EC 2.3.1.28) gene is expressed after transfection of L. enriettii with the hybrid plasmid. The expression of the CAT gene is dependent on the presence of sequences from the alpha-tubulin gene. The hybrid gene is also active in Leishmania braziliensis and Leishmania major.

MeSH Terms
Animals Base Sequence Chloramphenicol O-Acetyltransferase/genetics,metabolism Chromosome Deletion Gene Expression Genes Genes, Bacterial Leishmania mexicana/genetics Molecular Sequence Data Plasmids Recombinant Proteins/metabolism Transcription, Genetic Transfection Tubulin/genetics
Chemicals
Recombinant Proteins Tubulin Chloramphenicol O-Acetyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Laban A
Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115.
Wirth D F
References (35)
35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.23.9119
Published
1989-12-00
Pages
9119-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298445
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.23.9119 PMC Full Text View journal details
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