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PMID: 2602375 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleolar targeting signal of human T-cell leukemia virus type I rex-encoded protein is essential for cytoplasmic accumulation of unspliced viral mRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 24 ·1989-12-00 ·Pages 9798-802

Nosaka T, Siomi H, Adachi Y, Ishibashi M, Kubota S, Maki M, Hatanaka M

Abstract

The posttranscriptional regulator (rex) of human T-cell leukemia virus type I is known to be located predominantly in the cell nucleolus and to induce the accumulation of gag and env viral mRNAs. The N-terminal 19 amino acids of rex-encoded protein (Rex) has been shown to be sufficient to direct hybrid proteins to the cell nucleolus. We have studied the function of the nucleolar targeting signal (NOS) of rex by using full-length proviral DNA and mutant rex expression plasmids. Partial deletions of the NOS sequence abolished the accumulation of unspliced cytoplasmic mRNA, although the gene products of rex mutants were found in the nucleoplasm. These results indicate that NOS sequence, or nucleolar localization of Rex, is essential for Rex function.

MeSH Terms

Animals Blotting, Northern Cell Line Cell Nucleolus/metabolism Chloramphenicol O-Acetyltransferase/genetics Genetic Vectors Human T-lymphotropic virus 1/genetics Mutation Plasmids Proviruses/genetics,metabolism RNA Processing, Post-Transcriptional RNA, Messenger/genetics,metabolism RNA, Viral/genetics,metabolism Restriction Mapping Trans-Activators/analysis,genetics,metabolism Transfection

Chemicals

RNA, Messenger RNA, Viral Trans-Activators Chloramphenicol O-Acetyltransferase

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Nosaka T

Institute for Virus Research, Kyoto University, Japan.

Siomi H

Adachi Y

Ishibashi M

Kubota S

Maki M

Hatanaka M

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.86.24.9798

Published

1989-12-00

Pages

9798-802

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC298589

Subset

External Links

PubMed source DOI: 10.1073/pnas.86.24.9798 PMC Full Text View journal details

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Nucleolar targeting signal of human T-cell leukemia virus type I rex-encoded protein is essential for cytoplasmic accumulation of unspliced viral mRNA.

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