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PMID: 2643119 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and characterization of macrophage inflammatory protein 2.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 2 ·1989-01-00 ·Pages 612-6

Wolpe SD, Sherry B, Juers D, Davatelis G, Yurt RW, Cerami A

Abstract

In response to endotoxin, macrophages secrete a protein with a molecular mass of approximately 6000 Da and with an affinity for heparin. This protein, which we term "macrophage inflammatory protein 2," is a potent chemotactic agent for human polymorphonuclear leukocytes. In addition, subcutaneous administration of the monokine causes a localized inflammatory reaction. Partial N-terminal sequence data reveal similarity to a family of proteins, the archetype of which is platelet factor 4. Although macrophage inflammatory protein 2 is a distinct member of the platelet factor 4 family, its sequence is most closely related to that of the gro/KC gene product, which is expressed in transformed or platelet-derived growth factor-treated cells.

MeSH Terms
Amino Acid Sequence Animals Chemokine CXCL2 Chemokines, CXC Chemotactic Factors/analysis,immunology,isolation & purification Chemotaxis, Leukocyte Chromatography, Affinity Chromatography, Gel Hydrogen Peroxide/metabolism Immunoblotting Intercellular Signaling Peptides and Proteins Interleukin-8 Macrophages/analysis Mice Molecular Sequence Data Neutrophils/immunology
Chemicals
CXCL2 protein, human Chemokine CXCL2 Chemokines, CXC Chemotactic Factors Intercellular Signaling Peptides and Proteins Interleukin-8 Hydrogen Peroxide
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wolpe S D
Laboratory of Medical Biochemistry, Rockefeller University, New York, NY 10021-6399.
Sherry B
Juers D
Davatelis G
Yurt R W
Cerami A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.2.612
Published
1989-01-00
Pages
612-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286522
Subset
IM
Grants
NIAID NIH HHS · AI-12359 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.2.612 PMC Full Text View journal details
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