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PMID: 2890161 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Constitutive overexpression of a growth-regulated gene in transformed Chinese hamster and human cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 20 ·1987-10-00 ·Pages 7188-92

Anisowicz A, Bardwell L, Sager R

Abstract

Comparison by subtractive hybridization of mRNAs revealed a moderately abundant message in highly tumorigenic CHEF/16 cells present at very low levels in closely related nontumorigenic CHEF/18 cells. After cloning and sequencing the corresponding cDNA, computer comparison showed closest homology with the human connective tissue-activating peptide III (CTAP III). The human tumor cell cDNA hybridizing with the Chinese hamster clone was isolated, sequenced, and found to have closer similarity to the Chinese hamster gene than to CTAP III. Thus, the cloned cDNAs from Chinese hamster and human cells represent a different gene, named gro. Studies of its transcriptional regulation have shown that expression is tightly regulated by growth status in normal Chinese hamster and human cells and relaxed in the tumorigenic cells so far examined.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Division Cell Transformation, Neoplastic/genetics Chemokine CXCL1 Chemokines, CXC Cricetinae/genetics Cricetulus/genetics,metabolism DNA/genetics DNA, Neoplasm/genetics Fibroblasts/metabolism Gene Expression Regulation Humans Intercellular Signaling Peptides and Proteins Molecular Sequence Data Multigene Family Nucleic Acid Hybridization Peptides/genetics Poly A/biosynthesis Proto-Oncogene Proteins/biosynthesis,genetics,physiology Proto-Oncogenes RNA, Messenger/biosynthesis RNA, Neoplasm/biosynthesis Sequence Homology, Nucleic Acid Species Specificity Tumor Cells, Cultured/metabolism
Chemicals
CXCL1 protein, human Chemokine CXCL1 Chemokines, CXC DNA, Neoplasm Intercellular Signaling Peptides and Proteins Peptides Proto-Oncogene Proteins RNA, Messenger RNA, Neoplasm Poly A connective tissue-activating peptide DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Anisowicz A
Division of Cancer Genetics, Dana-Farber Cancer Institute, Boston, MA 02115.
Bardwell L
Sager R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.20.7188
Published
1987-10-00
Pages
7188-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299255
Subset
IM
Grants
NCI NIH HHS · CA39814 · United States
Databases
GENBANK
J03560, J03561
External Links
PubMed source DOI: 10.1073/pnas.84.20.7188 PMC Full Text View journal details
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