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PMID: 2647637 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Construction of a plasmid for expression of foreign epitopes as fusion proteins with subunit B of Escherichia coli heat-labile enterotoxin.

Infection and immunity ·Vol. 57 ·No. 4 ·1989-04-00 ·Pages 1347-50

Schödel F, Will H

Abstract

A novel vector (pFS2.2) for high-level expression of fusion polypeptides with the nontoxic subunit B (LT-B) of Escherichia coli heat-labile enterotoxin in Escherichia coli and salmonellae is presented. It carries the complete coding sequence of LT-B under lac promoter control and a universal polylinker site for the in-frame insertion of foreign genes at the LT-B gene 3' end. By using this vector, fusion proteins comprising parts of the human or woodchuck hepatitis B virus surface and nucleocapsid antigens are expressed in E. coli and salmonella.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Bacterial Toxins/genetics Base Sequence Enterotoxins/genetics Escherichia coli/genetics Escherichia coli Proteins Molecular Sequence Data Plasmids Recombinant Fusion Proteins/biosynthesis,isolation & purification Recombinant Proteins/biosynthesis,isolation & purification
Chemicals
Bacterial Proteins Bacterial Toxins Enterotoxins Escherichia coli Proteins Recombinant Fusion Proteins Recombinant Proteins heat-labile enterotoxin, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schödel F
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Will H
References (24)
24 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. A recombinant autoantigen derived from the human (U1) small nuclear RNP-specific 68-kd protein. Expression in Escherichia coli and serodiagnostic application.
    Arthritis Rheum. 1988 May;31(5):616-22 PMID: 2454119
  3. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
    J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli.
    Nature. 1979 Oct 25;281(5733):646-50 PMID: 399327
  6. Hepatitis B virus genes and their expression in E. coli.
    Nature. 1979 Dec 6;282(5739):575-9 PMID: 399329
  7. Amino acid sequence homology between cholera toxin and Escherichia coli heat-labile toxin.
    Nature. 1980 Dec 4;288(5790):499-501 PMID: 7003397
  8. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.
    Anal Biochem. 1981 Apr;112(2):295-8 PMID: 6266279
  9. Hepatocellular carcinoma and hepatitis B virus. A prospective study of 22 707 men in Taiwan.
    Lancet. 1981 Nov 21;2(8256):1129-33 PMID: 6118576
  10. Nucleotide sequence of a cloned woodchuck hepatitis virus genome: comparison with the hepatitis B virus sequence.
    J Virol. 1982 Jan;41(1):51-65 PMID: 7086958
  11. Cloning and molecular characterization of the B subunit of Escherichia coli heat-labile enterotoxin.
    Infect Immun. 1983 May;40(2):653-8 PMID: 6341243
  12. Construction of a potential live oral bivalent vaccine for typhoid fever and cholera-Escherichia coli-related diarrheas.
    Infect Immun. 1984 Nov;46(2):564-9 PMID: 6389350
  13. Hemagglutination assay of polypeptide coded by the pre-S region of hepatitis B virus DNA with monoclonal antibody: correlation of pre-S polypeptide with the receptor for polymerized human serum albumin in serums containing hepatitis B antigens.
    J Immunol. 1985 Feb;134(2):1212-6 PMID: 2981263
  14. Aromatic-dependent Salmonella dublin as a parenteral modified live vaccine for calves.
    Am J Vet Res. 1984 Nov;45(11):2231-5 PMID: 6395725
  15. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  16. Heat-labile enterotoxin in Escherichia coli. Kinetics of association of subunits into periplasmic holotoxin.
    J Biol Chem. 1985 Dec 15;260(29):16037-44 PMID: 3905802
  17. Antibodies to a synthetic peptide from the preS 120-145 region of the hepatitis B virus envelope are virus neutralizing.
    Vaccine. 1986 Mar;4(1):35-7 PMID: 2421497
  18. Monoclonal antibodies to Escherichia coli heat-labile enterotoxins: neutralising activity and differentiation of human and porcine LTs and cholera toxin.
    Med Biol. 1986;64(1):23-30 PMID: 3724258
  19. Oral immunization of mice with attenuated Salmonella enteritidis containing a recombinant plasmid which codes for production of the B subunit of heat-labile Escherichia coli enterotoxin.
    Infect Immun. 1986 Sep;53(3):685-92 PMID: 3527989
  20. A synthetic peptide vaccine involving the product of the pre-S(2) region of hepatitis B virus DNA: protective efficacy in chimpanzees.
    Proc Natl Acad Sci U S A. 1986 Dec;83(23):9174-8 PMID: 3466181
  21. Expression of the hepatitis B virus core gene in vitro and in vivo.
    J Virol. 1987 Oct;61(10):3109-13 PMID: 3625840
  22. Genetic and molecular basis for T- and B-cell recognition of hepatitis B viral antigens.
    Immunol Rev. 1987 Oct;99:71-103 PMID: 2445660
  23. Oral Salmonella typhimurium vaccine expressing circumsporozoite protein protects against malaria.
    Science. 1988 Apr 15;240(4850):336-8 PMID: 3281260
  24. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.4.1347-1350.1989
Published
1989-04-00
Pages
1347-50
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313276
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.57.4.1347-1350.1989 PMC Full Text View journal details
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