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PMID: 2654936 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Evidence for several higher order structural elements in ribosomal RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 9 ·1989-05-00 ·Pages 3119-22

Woese CR, Gutell RR

Abstract

Comparative analysis of small subunit ribosomal RNA sequences suggests the existence of two new higher order interactions: (i) a double-helical structure involving positions 505-507 and 524-526 (Escherichia coli numbering) and (ii) an interaction between the region of position 130 and the helix located approximately between positions 180 and 195. In the first of these, one of the strands of the helix exists in the bulge loop, and the other strand exists in the terminal loop of a previously recognized compound helix involving positions 500-545. Therefore, the new structure formally represents a pseudoknot. In the second, the insertion/deletion of a nucleotide in the vicinity of position 130 correlates with the length of the helix in the 180-195 region, the latter having a 3-base-pair stalk when the base in question is deleted and a stalk of approximately 10 pairs when it is inserted.

MeSH Terms

Animals Base Composition Base Sequence Escherichia coli/genetics Eubacterium/genetics Genetic Variation Mitochondria/analysis Mycoplasma/genetics Nucleic Acid Conformation RNA, Ribosomal RNA, Ribosomal, 16S Species Specificity

Chemicals

RNA, Ribosomal RNA, Ribosomal, 16S

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Woese C R

Department of Microbiology, University of Illinois, Urbana 61801.

Gutell R R

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26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.86.9.3119

Published

1989-05-00

Pages

3119-22

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC287076

Subset

Databases

GENBANK

M22441

External Links

PubMed source DOI: 10.1073/pnas.86.9.3119 PMC Full Text View journal details

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Evidence for several higher order structural elements in ribosomal RNA.

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