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PMID: 3116271 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Drosophila mitochondrial DNA: conserved sequences in the A + T-rich region and supporting evidence for a secondary structure model of the small ribosomal RNA.

Journal of molecular evolution ·Vol. 25 ·No. 2 ·1987-00-00 ·Pages 116-25

Clary DO, Wolstenholme DR

Abstract

The sequence of a segment of the Drosophila virilis mitochondrial DNA (mtDNA) molecule that contains the A + T-rich region, the small rRNA gene, the tRNA(f-met), tRNA(gln), and tRNA(ile) genes, and portions of the ND2 and tRNA(val) genes is presented and compared with the corresponding segment of the D. yakuba mtDNA molecule. The A + T-rich regions of D. virilis and D. yakuba contain two correspondingly located sequences of 49 and 276/274 nucleotides that appear to have been conserved during evolution. In each species the replication origin of the mtDNA molecule is calculated to lie within a region that overlaps the larger conserved sequence, and within this overlap is found a potential hairpin structure. Substitutions between the larger conserved sequences of the A + T-rich regions, the small mt-rRNA genes, and the ND2 genes are biased in favor of transversions, 71-97% of which are A----T changes. There is a 13.8 times higher frequency of nucleotide differences between the 5' halves than between the 3' halves of the D. virilis and D. yakuba small mt-rRNA genes. Considerations of the effects of observed substitutions and deletion/insertions on possible nucleotide pairing within the small mt-rRNA genes of D. virilis and D. yakuba strongly support the secondary structure model for the Drosophila small mt-rRNA that we previously proposed.

MeSH Terms
Adenine Animals Base Composition Base Sequence Biological Evolution DNA, Mitochondrial/genetics Drosophila/genetics Molecular Sequence Data Nucleic Acid Conformation RNA, Ribosomal/genetics Species Specificity Thymine
Chemicals
DNA, Mitochondrial RNA, Ribosomal Adenine Thymine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clary D O
Department of Biology, University of Utah, Salt Lake City 84112.
Wolstenholme D R
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF02101753
Published
1987-00-00
Pages
116-25
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
Grants
NIGMS NIH HHS · GM-18375 · United States
Databases
GENBANK
X05915
External Links
PubMed source DOI: 10.1007/BF02101753 View journal details
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