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PMID: 2656254 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A second proteinase encoded by a plant potyvirus genome.

The EMBO journal ·Vol. 8 ·No. 2 ·1989-02-00 ·Pages 365-70

Carrington JC, Cary SM, Parks TD, Dougherty WG

Abstract

The RNA genome of tobacco etch virus (TEV) encodes a large polyprotein precursor that is processed to mature proteins by virus-specific proteinases. Cleavage sites located within the carboxyl-terminal two-thirds of the polyprotein are processed by a TEV-encoded 49 kd proteinase, while the enzyme(s) responsible for cleaving the remaining sites has not been found. In this study, a second TEV-encoded proteinase has been identified based on cell-free expression of defined RNA transcripts. The boundaries of this proteinase have been delineated by deletion analysis and site-directed mutagenesis. The proteolytically active domain has been localized to the carboxyl-terminal half of the 56 kd aphid-transmission helper component. A cleavage site that is recognized by this proteinase has been identified in the polyprotein adjacent to the carboxyl-terminus of the enzyme, and the proteinase appears to cleave by an autocatalytic mechanism. Proteolysis in vitro occurs between a Gly-Gly dipeptide as determined by radiochemical sequencing at the amino-terminus of the proteolytic product.

MeSH Terms
Amino Acid Sequence Binding Sites Endopeptidases/genetics,metabolism Genes, Viral Molecular Sequence Data Mutation Plant Viruses/genetics,metabolism Protein Precursors/metabolism Protein Processing, Post-Translational
Chemicals
Protein Precursors Endopeptidases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Carrington J C
Department of Biology, Texas A&M University, College Station 77843.
Cary S M
Parks T D
Dougherty W G
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19 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-02-00
Pages
365-70
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400815
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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