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PMID: 6384934 Published · ppublish English Comparative Study Journal Article

Similarity in gene organization and homology between proteins of animal picornaviruses and a plant comovirus suggest common ancestry of these virus families.

Nucleic acids research ·Vol. 12 ·No. 18 ·1984-09-25 ·Pages 7251-67

Argos P, Kamer G, Nicklin MJ, Wimmer E

Abstract

The amino acid sequences deduced from the nucleic acid sequences of several animal picornaviruses and cowpea mosaic virus (CPMV), a plant virus, were compared. Good homology was found between CPMV and the picornaviruses in the region of the picornavirus 2C (P2-X protein), VPg, 3C pro (proteinase) and 3D pol (RNA polymerase) regions. The CPMV B genome was found to have a similar gene organization to the picornaviruses. A comparison of the 3C pro (proteinase) regions of all of the available picornavirus sequences and CPMV allowed us to identify residues that are completely conserved; of these only two residues, Cys-147 and His-161 (poliovirus proteinase) could be the reactive residues of the active site of a proteinase with analogous mechanism to a known proteinase. We conclude that the proteinases encoded by these viruses are probably cysteine proteinases, mechanistically related, but not homologous to papain.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Evolution DNA-Directed RNA Polymerases/genetics Genes Genes, Viral Mosaic Viruses/genetics Peptide Hydrolases/genetics Picornaviridae/genetics Species Specificity Viral Proteins/genetics
Chemicals
Viral Proteins DNA-Directed RNA Polymerases Peptide Hydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Argos P
Kamer G
Nicklin M J
Wimmer E
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43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.18.7251
Published
1984-09-25
Pages
7251-67
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320155
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.18.7251 PMC Full Text View journal details
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