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PMID: 2659537 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and sequencing of a Treponema pallidum gene encoding a 31.3-kilodalton endoflagellar subunit (FlaB2).

Infection and immunity ·Vol. 57 ·No. 7 ·1989-07-00 ·Pages 2166-72

Pallesen L, Hindersson P

Abstract

A library of Treponema pallidum DNA was established in the direct selection vector pUN121. Six clones carrying a gene coding for a 33-kilodalton T. pallidum flagellum subunit were identified by colony hybridization with an oligodeoxynucleotide probe based on the N-terminal amino acid sequence of this subunit. An open reading frame of 286 amino acids with the expected N-terminal sequence and absence of cysteine residues was identified. The deduced protein had a calculated molecular weight of 31.3 kilodaltons. We propose to name this flagellar subunit FlaB2. FlaB2 shows a significant amino acid homology with flagellins of several remotely related bacterial species. This homology was most pronounced corresponding to the C-terminal and N-terminal parts of the protein, whereas the central region was variable.

MeSH Terms
Amino Acid Sequence Antigens, Bacterial/genetics,isolation & purification Base Sequence Cloning, Molecular/methods Flagella/analysis,physiology Genes Genes, Bacterial Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid Treponema pallidum/genetics,isolation & purification
Chemicals
Antigens, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pallesen L
Department of Treponematoses, Statens Seruminstitut, Copenhagen, Denmark.
Hindersson P
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28 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.7.2166-2172.1989
Published
1989-07-00
Pages
2166-72
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313856
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.57.7.2166-2172.1989 PMC Full Text View journal details
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