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PMID: 2662183 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Rat glucokinase gene: structure and regulation by insulin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 13 ·1989-07-00 ·Pages 4838-42

Magnuson MA, Andreone TL, Printz RL, Koch S, Granner DK

Abstract

The glucokinase gene is 15.5-kilobases long, appears to be present as a single copy, and contains 10 exons that range in size from 96 to 977 base pairs. The transcription start site was located 127 nucleotides upstream from the translation initiation codon. The 5' flanking DNA contains several regions similar to defined promoter elements. These include a probable "TATA box," an Sp1 binding site, and several elements related to liver-specific gene expression. In addition, we determined that transcription of the glucokinase gene increased at least 20-fold when diabetic rats were treated with insulin for 2 hr.

MeSH Terms
Animals Base Sequence Cell Nucleus/metabolism Diabetes Mellitus, Experimental/drug therapy,enzymology,genetics Exons Gene Expression Regulation/drug effects Genes/drug effects Glucokinase/genetics Insulin/pharmacology,therapeutic use Introns Liver/enzymology Male Molecular Sequence Data Rats Rats, Inbred Strains Restriction Mapping Transcription, Genetic/drug effects
Chemicals
Insulin Glucokinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Magnuson M A
Department of Molecular Physiology and Biophysics, Vanderbilt University Medical School, Nashville 37232.
Andreone T L
Printz R L
Koch S
Granner D K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.13.4838
Published
1989-07-00
Pages
4838-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297510
Subset
IM
Grants
NIDDK NIH HHS · DK 07061 · United States
NIDDK NIH HHS · DK 35107 · United States
Databases
GENBANK
M24943, M24944, M24945, M24946, M24947, M24948, M24949, M24950, M24951, M24952
External Links
PubMed source DOI: 10.1073/pnas.86.13.4838 PMC Full Text View journal details
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