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PMID: 26655834 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isoform diversity in the Arp2/3 complex determines actin filament dynamics.

Nature cell biology ·Vol. 18 ·No. 1 ·2016-01-00 ·Pages 76-86

Abella JV, Galloni C, Pernier J, Barry DJ, Kjær S, Carlier MF, Way M

Abstract

The Arp2/3 complex consists of seven evolutionarily conserved subunits (Arp2, Arp3 and ARPC1-5) and plays an essential role in generating branched actin filament networks during many different cellular processes. In mammals, however, the ARPC1 and ARPC5 subunits are each encoded by two isoforms that are 67% identical. This raises the possibility that Arp2/3 complexes with different properties may exist.  We found that Arp2/3 complexes containing ARPC1B and ARPC5L are significantly better at promoting actin assembly than those with ARPC1A and ARPC5, both in cells and in vitro. Branched actin networks induced by complexes containing ARPC1B or ARPC5L are also disassembled ∼2-fold slower than those formed by their counterparts. This difference reflects the ability of cortactin to stabilize ARPC1B- and ARPC5L- but not ARPC1A- and ARPC5-containing complexes against coronin-mediated disassembly. Our observations demonstrate that the Arp2/3 complex in higher eukaryotes is actually a family of complexes with different properties.

MeSH Terms
Actin Cytoskeleton/metabolism Actin-Related Protein 2/metabolism Actin-Related Protein 3/metabolism Angiopoietin-Like Protein 2 Angiopoietin-like Proteins Angiopoietins/metabolism Animals Cell Line Cortactin/metabolism Humans Mice Microfilament Proteins/metabolism Protein Isoforms/chemistry,metabolism
Chemicals
ACTR2 protein, human ACTR3 protein, human Actin-Related Protein 2 Actin-Related Protein 3 Actr3 protein, mouse Angiopoietin-Like Protein 2 Angiopoietin-like Proteins Angiopoietins Angptl2 protein, mouse CTTN protein, human Cortactin Cttn protein, mouse Microfilament Proteins Protein Isoforms coronin proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Abella Jasmine V G
Cellular Signalling and Cytoskeletal Function, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, 44 Lincoln's Inn Fields, London, WC2A 3LY, UK.
Galloni Chiara
Cellular Signalling and Cytoskeletal Function, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, 44 Lincoln's Inn Fields, London, WC2A 3LY, UK.
Pernier Julien
Laboratoire d'Enzymologie et Biochimie Structurale, I2BC, CNRS, 91198 Gif-sur-Yvette, France.
Barry David J
Cellular Signalling and Cytoskeletal Function, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, 44 Lincoln's Inn Fields, London, WC2A 3LY, UK.
Kjær Svend
The Structural Biology Science Technology Platform, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, 44 Lincoln's Inn Fields, London, WC2A 3LY, UK.
Carlier Marie-France
Laboratoire d'Enzymologie et Biochimie Structurale, I2BC, CNRS, 91198 Gif-sur-Yvette, France.
Way Michael
Cellular Signalling and Cytoskeletal Function, The Francis Crick Institute, Lincoln's Inn Fields Laboratory, 44 Lincoln's Inn Fields, London, WC2A 3LY, UK.
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Article Info
Journal
Nature cell biology
Abbr.
Nat Cell Biol
ISSN
1476-4679
DOI
10.1038/ncb3286
Published
2016-01-00
Epub
2015-00-14
Pages
76-86
Language
English
Region
England
NLM ID
100890575
Subset
IM
Grants
Canadian Institutes of Health Research · Canada
Cancer Research UK · United Kingdom
Corrections
CommentIn
PMID: 26693915
External Links
PubMed source DOI: 10.1038/ncb3286 View journal details
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