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PMID: 2666404 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Sequence and structure of the yeast galactose transporter.

Journal of bacteriology ·Vol. 171 ·No. 8 ·1989-08-00 ·Pages 4486-93

Szkutnicka K, Tschopp JF, Andrews L, Cirillo VP

Abstract

The previously cloned GAL2 gene of the Saccharomyces cerevisiae galactose transporter has been sequenced. The nucleotide sequence predicts a protein with 574 amino acids (Mr, 63,789). Hydropathy plots suggest that there are 12 membrane-spanning segments. The galactose transporter shows both sequence and structural homology with a superfamily of sugar transporters which includes the human HepG2-erythrocyte and fetal muscle glucose transporters, the rat brain and liver glucose transporters, the Escherichia coli xylose and arabinose permeases, and the S. cerevisiae glucose, maltose, and galactose transporters. Sequence and structural motifs at the N-terminal and C-terminal regions of the proteins support the view that the genes of this superfamily arose by duplication of a common ancestral gene. In addition to the sequence homology and the presence of the 12 membrane-spanning segments, the members of the superfamily show characteristic lengths and distributions of the charged, hydrophilic connecting loops. There is indirect evidence that the transporter is an N-glycoprotein. However, its only N-glycosylation site occurs in a charged, hydrophilic segment. This could mean that this segment is part of a hydrophilic channel in the membrane. The transporter has a substrate site for the cyclic AMP-dependent protein kinase which may be a target of catabolite inactivation. The transporter lacks a strong sequence enriched for proline (P), glutamate (E), aspartate, serine (S), and threonine (T) and flanked by basic amino acids (PEST sequence) even though it has a short half-life. Mechanisms for converting the poor PEST to a possible PEST sequence are considered. Like the other members of the superfamily, the galactose transporter lacks a signal sequence.

MeSH Terms
Amino Acid Sequence Base Sequence Calcium-Binding Proteins Carrier Proteins/genetics Cell Membrane/metabolism Cloning, Molecular Galactose/metabolism Genes Genes, Fungal Molecular Sequence Data Monosaccharide Transport Proteins Periplasmic Binding Proteins Protein Conformation Saccharomyces cerevisiae/genetics,metabolism Sequence Homology, Nucleic Acid
Chemicals
Calcium-Binding Proteins Carrier Proteins Monosaccharide Transport Proteins Periplasmic Binding Proteins galactose-binding protein Galactose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Szkutnicka K
Department of Biochemistry, State University of New York, Stony Brook 11794.
Tschopp J F
Andrews L
Cirillo V P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.8.4486-4493.1989
Published
1989-08-00
Pages
4486-93
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210229
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.8.4486-4493.1989 PMC Full Text View journal details
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