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PMID: 2669628 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SHV-5, a novel SHV-type beta-lactamase that hydrolyzes broad-spectrum cephalosporins and monobactams.

Antimicrobial agents and chemotherapy ·Vol. 33 ·No. 6 ·1989-06-00 ·Pages 951-6

Gutmann L, Ferré B, Goldstein FW, Rizk N, Pinto-Schuster E, Acar JF, Collatz E

Abstract

SHV-5 (pI 8.2), a novel broad-spectrum beta-lactamase encoded by a ca. 150-kilobase plasmid, was found in Klebsiella pneumoniae 160. SHV-5 beta-lactamase caused decreased susceptibility to most penicillins, cephalosporins, and monobactams, except imipenem and compounds which have a C6 or C7 alpha-methoxy substituent. beta-Lactamase inhibitors (clavulanic acid, sulbactam, and tazobactam) inhibited its activity and showed a synergistic effect when associated with different hydrolyzable beta-lactam compounds. Hybridization studies suggested that this enzyme may be related to, or derived from, the SHV enzyme. Increased MICs of cephamycins and temocillin associated with a decreased synergistic effect of the inhibitors on K. pneumoniae 160 might be linked to a decrease in two outer membrane proteins.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacterial Outer Membrane Proteins/metabolism Cephalosporins/metabolism DNA, Bacterial/biosynthesis Klebsiella pneumoniae/enzymology Microbial Sensitivity Tests Monobactams/metabolism Nucleic Acid Hybridization Plasmids beta-Lactamase Inhibitors beta-Lactamases/biosynthesis,metabolism
Chemicals
Anti-Bacterial Agents Bacterial Outer Membrane Proteins Cephalosporins DNA, Bacterial Monobactams beta-Lactamase Inhibitors beta-Lactamases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Gutmann L
Laboratoire de Microbiologie Médicale, Hospital Broussais, Paris, France.
Ferré B
Goldstein F W
Rizk N
Pinto-Schuster E
Acar J F
Collatz E
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.33.6.951
Published
1989-06-00
Pages
951-6
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC284261
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.33.6.951 PMC Full Text View journal details
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