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PMID: 3879659 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evolution of plasmid-coded resistance to broad-spectrum cephalosporins.

Antimicrobial agents and chemotherapy ·Vol. 28 ·No. 2 ·1985-08-00 ·Pages 302-7

Kliebe C, Nies BA, Meyer JF, Tolxdorff-Neutzling RM, Wiedemann B

Abstract

A clinical isolate of Klebsiella ozaenae with transferable resistance to broad-spectrum cephalosporins produces a beta-lactamase determined by plasmid pBP60. The beta-lactamase had the same isoelectric point as SHV-1 (7.6). From heteroduplex analysis, an extensive homology between the two bla genes could be deduced; therefore, the new beta-lactamase was designated SHV-2. Enzymatic studies revealed that SHV-2 was able to hydrolyze broad-spectrum cephalosporins due to an increased affinity of these compounds for the enzyme. The assumption that SHV-2 is a natural mutant of SHV-1 was strongly supported by the isolation of a laboratory mutant of SHV-1 that showed activities similar to those of SHV-2.

MeSH Terms
Cephalosporins/metabolism,pharmacology Cloning, Molecular DNA, Bacterial/genetics Drug Resistance, Microbial Genes Genes, Bacterial Klebsiella/drug effects,genetics Mutation Plasmids Sequence Homology, Nucleic Acid beta-Lactamases/genetics,metabolism
Chemicals
Cephalosporins DNA, Bacterial beta-Lactamases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kliebe C
Nies B A
Meyer J F
Tolxdorff-Neutzling R M
Wiedemann B
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.28.2.302
Published
1985-08-00
Pages
302-7
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC180236
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.28.2.302 PMC Full Text View journal details
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