Home LiteratureArticle Details
PMID: 2671649 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning and nucleotide sequence of the Salmonella typhimurium LT2 gnd gene and its homology with the corresponding sequence of Escherichia coli K12.

Molecular & general genetics : MGG ·Vol. 217 ·No. 1 ·1989-05-00 ·Pages 182-4

Reeves P, Stevenson G

Abstract

The complete nucleotide sequence of the Salmonella strain LT2 gnd gene for 6-phosphogluconate dehydrogenase was determined. The gene contains 1404 bases and encodes a 468 amino acid polypeptide, which is the same as for Escherichia coli K12. The DNA sequence shows 14.8% difference between the two and the amino acid sequence 3.6% difference. Changes are mostly in the third codon base and most of the amino acid changes are conservative.

MeSH Terms
Amino Acid Sequence Base Composition Base Sequence Cloning, Molecular Codon DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Phosphogluconate Dehydrogenase/genetics Plasmids Restriction Mapping Salmonella typhimurium/genetics Sequence Homology, Nucleic Acid
Chemicals
Codon DNA, Bacterial Phosphogluconate Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reeves P
Department of Microbiology, University of Sydney, Australia.
Stevenson G
References (12)
12 references, click to expand
  1. DNA sequence of the Escherichia coli gene, gnd, for 6-phosphogluconate dehydrogenase.
    Gene. 1984 Mar;27(3):253-64 PMID: 6329905
  2. The rate of synonymous substitution in enterobacterial genes is inversely related to codon usage bias.
    Mol Biol Evol. 1987 May;4(3):222-30 PMID: 3328816
  3. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  4. Cloning part of the region encoding biosynthetic enzymes for surface antigen (O-antigen) of Salmonella typhimurium.
    Mol Gen Genet. 1986 Apr;203(1):172-6 PMID: 2423848
  5. MBIS--an integrated system for the retrieval and analyses of sequence data from nucleic acids and proteins.
    Nucleic Acids Res. 1986 Jan 10;14(1):265-72 PMID: 3753768
  6. Rapid and efficient cosmid cloning.
    Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 PMID: 6269067
  7. Evolution in bacteria: evidence for a universal substitution rate in cellular genomes.
    J Mol Evol. 1987;26(1-2):74-86 PMID: 3125340
  8. Comparative nucleotide sequence analysis of growth-rate-regulated gnd alleles from natural isolates of Escherichia coli and from Salmonella typhimurium LT-2.
    J Bacteriol. 1988 Jan;170(1):372-9 PMID: 3275621
  9. The codon Adaptation Index--a measure of directional synonymous codon usage bias, and its potential applications.
    Nucleic Acids Res. 1987 Feb 11;15(3):1281-95 PMID: 3547335
  10. Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes.
    J Mol Biol. 1981 Feb 15;146(1):1-21 PMID: 6167728
  11. Growth-rate-dependent expression and cloning of gnd alleles from natural isolates of Escherichia coli.
    J Bacteriol. 1988 Jan;170(1):365-71 PMID: 2826398
  12. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
Published
1989-05-00
Pages
182-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X15651
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]