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PMID: 2675782 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Immunolocalization of matrix metalloproteinase 3 (stromelysin) in rheumatoid synovioblasts (B cells): correlation with rheumatoid arthritis.

Annals of the rheumatic diseases ·Vol. 48 ·No. 8 ·1989-08-00 ·Pages 645-53

Okada Y, Takeuchi N, Tomita K, Nakanishi I, Nagase H

Abstract

Metalloproteinases produced by connective tissue cells may play a key part in the destruction of joints in rheumatoid arthritis. Matrix metalloproteinase 3 (MMP-3; stromelysin) capable of degrading cartilage proteoglycans and type IX collagen and of activating procollagenase was immunolocalised in hyperplastic synovial lining cells in rheumatoid synovium, but not in the cells of normal synovium. Cells responsible for synthesis of MMP-3 have the phenotype of synovioblasts (B cells) by immunoelectron microscopy, but not of phagocytic synovial macrophages (A cells). Cultured monolayer of rheumatoid synovial cells synthesises MMP-3 only under treatment with macrophage conditioned medium. Immunolocalisation of MMP-3 in rheumatoid synovium and cultured synovial cells was possible when the specimens were treated with a monovalent ionophore, monensin. These results suggest that MMP-3 is synthesised and secreted continuously without storage from hyperplastic synovioblasts stimulated by factor(s) derived from activated macrophages present in the synovium.

MeSH Terms
Arthritis, Rheumatoid/enzymology,immunology B-Lymphocytes/enzymology,immunology Cells, Cultured Fluorescent Antibody Technique Humans Matrix Metalloproteinase 3 Metalloendopeptidases/analysis,biosynthesis,immunology Microscopy, Electron Synovial Membrane/enzymology,immunology
Chemicals
Metalloendopeptidases Matrix Metalloproteinase 3
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Okada Y
Department of Pathology, School of Medicine, Kanazawa University, Ishikawa, Japan.
Takeuchi N
Tomita K
Nakanishi I
Nagase H
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Article Info
Journal
Annals of the rheumatic diseases
Abbr.
Ann Rheum Dis
ISSN
0003-4967
DOI
10.1136/ard.48.8.645
Published
1989-08-00
Pages
645-53
Language
English
Region
England
NLM ID
0372355
PMCID
PMC1003840
Subset
IM
Grants
NIAMS NIH HHS · AR 39189 · United States
External Links
PubMed source DOI: 10.1136/ard.48.8.645 PMC Full Text View journal details
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